Expression of apoptosis-related factors in muscles infected with Trichinella spiralis
Expression of apoptosis-related factors in muscles infected with Trichinella spiralis
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DOI：
10.1017/s0031182003004530
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发表时间：
2004-03-01
期刊：
影响因子：
2.4
通讯作者：
Takahashi, Y
中科院分区：
文献类型：
--
作者：
Boonmars, T;Wu, Z;Takahashi, Y
研究发现，在旋毛虫感染肌肉中，线粒体凋亡相关基因(Bcl2相关蛋白X，Bax；凋亡蛋白激活因子1，APAF-1；Caspase9，丝氨酸/苏氨酸蛋白激酶，PKB)在包裹过程中表达上调。对被膜的显微解剖和随后的逆转录聚合酶链式反应(RT-PCR)证实，这些基因的表达仅限于哺乳细胞。免疫细胞化学显示促凋亡因子(Bax、APAF-1和Caspase9)主要表达在嗜碱性细胞质(感染肌肉细胞起源)和抗凋亡因子(PKB)表达在嗜酸性细胞质(卫星细胞起源)。电子显微镜显示，新生幼虫入侵后，肌细胞中原有的线粒体立即肿胀消失，但细胞质中出现了新的较小的线粒体。嗜碱性细胞质内可见核碎裂和凝聚，已知死亡。综上所述，这些结果表明，受感染的肌肉细胞通过新形成的线粒体中的因素触发的凋亡过程进行转化但死亡。抗细胞凋亡因子可能有助于嗜酸性细胞质的存活，以确保护士细胞的功能。
found that the expression of mitochondrial apoptosis related genes (Bcl-2 associated protein X, BAX; apoptotic protease activating factor 1, Apaf-1; Caspase 9 and serine/threonine protein kinase, PKB) is elevated in Trichinella spiralis-infected muscles during encapsulation. Micro-dissection of the capsule and subsequent reverse transcription poly-merase chain reaction (RT-PCR) confirmed that the expressions of these genes are restricted to the nurse cell. lmmunocytochemistry revealed that pro-apoptosis factor (BAX, Apaf-1 and Caspase 9) are predominantly expressed in the basophilic cytoplasm (infected muscle cell origin) and anti-apoptosis factor (PKB) in the eosinophilic cytoplasm (satellite cell origin) of the nurse cell. Electron microscopy revealed that the pre-existing mitochondria in the muscle cells became swollen and disappeared immediately after newborn larva invasion, but new mitochondria of smaller size appeared in the cytoplasm. Nuclear fragmentation and condensation were observed in basophilic cytoplasm which is known to die. Together, the results suggest that the infected muscle cells transform but die through the process of apoptosis which is triggered by factors from the newly formed mitochondria. The anti-apoptosis factor may help the eosinophilic cytoplasm with its survival to ensure nurse cell function.