Oxygen concentration imaging in a single living cell using phosphorescence lifetime of Pt-porphyrin
Oxygen concentration imaging in a single living cell using phosphorescence lifetime of Pt-porphyrin
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DOI：
10.1142/s1088424607000205
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发表时间：
2007-01-01
影响因子：
1.5
通讯作者：
Okura, Ichiro
中科院分区：
文献类型：
--
作者：
Saito, Takashi;Asakura, Noriyuki;Okura, Ichiro
开发了在显微镜下基于磷光寿命的单个活细胞内的氧浓度成像系统。使用配备脉冲 Nd:YAG 激光器 (532 nm) 的荧光显微镜和配备门控成像增强器的 CCD 相机。当细胞与磷光化合物一起孵育时，四（羧基苯基）-卟啉铂（PtTCPP）被掺入并定位在细胞中。由于磷光强度不仅取决于氧等猝灭剂的浓度，还取决于磷光分子的浓度，因此通过磷光寿命来测量氧浓度。为了测量氧浓度图像，在显微镜下观察磷光强度的时间依赖性。磷光强度衰减服从一级动力学，通过单指数衰减曲线的寿命获得单细胞内的氧浓度成像。版权所有 (c) 2007 卟啉和酞菁协会。
An oxygen concentration imaging system inside a single living cell, based on the phosphorescence lifetime, under a microscope was developed. A fluorescence microscope equipped with a pulsed Nd:YAG laser (532 nm) and a CCD camera equipped with a gated imaging intensifier was used. When the cell was incubated with the phosphorescent compound, platinum tetra(carboxyphenyl)-porphyrin (PtTCPP) was incorporated and localized in the cell. As the phosphorescence intensity depends not only on the concentration of a quencher such as oxygen but also on the concentration of phosphorescent molecules, the oxygen concentration was measured by the phosphorescence lifetime. To measure the oxygen concentration image, the time dependence of the phosphorescence intensity was observed under a microscope. The phosphorescence intensity decay obeyed first order kinetics and the oxygen concentration imaging in the single cell was obtained by the lifetime of a single exponential decay curve. Copyright (c) 2007 Society of Porphyrins & Phthalocyanines.