Antibacterial effect of rose bengal against colistin-resistant gram-negative bacteria.
Antibacterial effect of rose bengal against colistin-resistant gram-negative bacteria.
复制标题
孟加拉玫瑰对粘菌素耐药革兰氏阴性菌的抗菌作用。
DOI：
10.1038/s41429-023-00622-1
复制
发表时间：
2023
期刊：
影响因子：
--
通讯作者：
Rodrigues,DominicJ
中科院分区：
文献类型：
--
作者：
Kurosu,Michio;Mitachi,Katsuhiko;Pershing,EdwardV;Horowitz,BruceD;Wachter,EricA;Lacey3rd,JohnW;Ji,Yinduo;Rodrigues,DominicJ
革兰氏阴性菌耐药性的增加在全世界范围内带来了严重的健康问题。尽管新一代β-内酰胺类、氨基糖苷类和氟喹诺酮类药物的开发取得了显着进展，但治疗多重耐药革兰氏阴性细菌感染仍然具有挑战性。粘菌素（多粘菌素E）是治疗多种耐药革兰氏阴性菌最有效的抗生素之一，在临床上已被用作最后的选择。然而，可转移基因 mcr-1 通过编码修饰细菌膜脂质 A 的磷酸乙醇胺转移酶而赋予粘菌素耐药性，其快速传播威胁着粘菌素治疗耐药细菌感染的功效。铜绿假单胞菌、鲍曼不动杆菌和肺炎克雷伯菌的粘菌素抗性菌株通常会降低其对其他抗革兰氏阴性细菌制剂的敏感性。因此，迫切需要有效对抗粘菌素耐药菌株的药物或防止在治疗期间获得粘菌素耐药性的方法。为了对收集的小分子进行基于细胞的筛选，我们产生了大肠杆菌素耐药菌株。大肠杆菌，A.鲍曼尼，K.肺炎杆菌，P.铜绿假单胞菌，和S.鼠伤寒肠杆菌。通过内部 MIC 测定筛选，我们发现玫瑰红（4,5,6,7-四氯-2',4',5',7'-四碘荧光素）是唯一在低浓度光照条件下对这些菌株表现出独特杀菌活性的分子。本文报道了医药级孟加拉玫瑰对粘菌素耐药的革兰氏阴性菌的抗菌活性。
Increasing drug resistance in Gram-negative bacteria presents significant health problems worldwide. Despite notable advances in the development of a new generation of β-lactams, aminoglycosides, and fluoroquinolones, it remains challenging to treat multi-drug resistant Gram-negative bacterial infections. Colistin (polymyxin E) is one of the most efficacious antibiotics for the treatment of multiple drug-resistant Gram-negative bacteria and has been used clinically as a last-resort option. However, the rapid spread of the transferable gene,mcr-1which confers colistin resistance by encoding a phosphoethanolamine transferase that modifies lipid A of the bacterial membrane, threatens the efficacy of colistin for the treatment of drug-resistant bacterial infections. Colistin-resistant strains ofPseudomonas aeruginosa, Acinetobacter baumannii, andKlebsiella pneumoniaeoften reduce their susceptibility to other anti-Gram-negative bacterial agents. Thus, drugs effective against colistin-resistant strains or methods to prevent the acquisition of colistin-resistance during treatment are urgently needed. To perform cell-based screenings of the collected small molecules, we have generated colistin-resistant strains ofE. coli,A. baumannii,K. pneumoniae,P. aeruginosa, andS. entericaTyphimurium. In-house MIC assay screenings, we have identified that rose bengal (4,5,6,7-tetrachloro-2’,4’,5’,7’-tetraiodofluorescein) is the only molecule that displays unique bactericidal activity against these strains at low concentrations under illumination conditions. This article reports the antibacterial activity of a pharmaceutical-grade rose bengal against colistin-resistant Gram-negative bacteria.