High-resolution mapping of QTL for fatty acid composition in soybean using specific-locus amplified fragment sequencing.
High-resolution mapping of QTL for fatty acid composition in soybean using specific-locus amplified fragment sequencing.
复制标题
使用特定位点扩增片段测序对大豆脂肪酸组成的 QTL 进行高分辨率定位
DOI：
10.1007/s00122-017-2902-8
复制
发表时间：
2017-07
期刊：
影响因子：
--
通讯作者：
Sun J
中科院分区：
文献类型：
--
作者：
Li B;Fan S;Yu F;Chen Y;Zhang S;Han F;Yan S;Wang L;Sun J
本研究构建了一张由3541个标记组成的高密度连锁图谱，并鉴定了26个稳定的QTL，其中包括9个新位点，用于大豆脂肪酸组成的分析。本研究利用栽培大豆品种鲁黑豆2号与南汇早杂交获得的200个重组自交系，以及通过特异位点扩增片段测序（SLAF-seq）开发的SNP标记，构建了高密度遗传连锁图谱。该图谱由20个连锁群上的3541个标记组成，遗传距离为2534.42cM，相邻标记间的平均距离为0.72cM。包含性复合区间作图揭示了5种脂肪酸的26个稳定QTL，解释了0.4-37.0%的表型变异。在这些QTL中，有9个是新位点（qLA 1、qLNA 2_1、qPA 4_1、qLA 4_1、qPA 6_1、qSA 12_1、qPA 16_1、qOA 18_1和qFA 19_1）。这些稳定的QTL包含3个脂肪酸合成基因（GmFabG、GmACP和GmFAD 8）和66个编码脂类相关转录因子的基因。这些稳定的QTL和紧密连锁的SNP标记可用于大豆育种中的标记辅助选择。
Key messageWe constructed a high-density linkage map comprising 3541 markers developed by specific-locus amplified fragment sequencing, and identified 26 stable QTL including nine novel loci, for fatty acid composition in soybean.AbstractSoybean oil quality and stability are mainly determined by the fatty acid composition of the seed. In the present study, we constructed a high-density genetic linkage map using 200 recombinant inbred lines derived from a cross between cultivated soybean varieties Luheidou2 and Nanhuizao, and SNP markers developed by specific-locus amplified fragment sequencing (SLAF-seq). This map comprises 3541 markers on 20 linkage groups and spans a genetic distance of 2534.42 cM, with an average distance of 0.72 cM between adjacent markers. Inclusive composite interval mapping revealed 26 stable QTL for five fatty acids, explaining 0.4–37.0% of the phenotypic variance for individual fatty acids across environments. Of these QTL, nine are novel loci (qLA1,qLNA2_1,qPA4_1,qLA4_1,qPA6_1,qSA12_1,qPA16_1,qOA18_1, andqFA19_1). These stable QTL harbor three fatty acid biosynthesis genes (GmFabG,GmACP, andGmFAD8), and 66 genes encoding lipid-related transcription factors. These stable QTL and tightly linked SNP markers can be used for marker-assisted selection in soybean breeding programs.