Development of immune response to cow's milk proteins in infants receiving cow's milk or hydrolyzed formula
Development of immune response to cow's milk proteins in infants receiving cow's milk or hydrolyzed formula
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DOI：
10.1016/s0091-6749(95)70229-6
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发表时间：
1995-12-01
影响因子：
14.2
通讯作者：
Akerblom, HK
中科院分区：
文献类型：
--
作者：
Vaarala, O;Saukkonen, T;Akerblom, HK
背景：人类对口服抗原的体液和细胞免疫反应的发展尚不清楚，尽管抗原给药已被认为是治疗超敏性疾病和自身免疫性疾病的一种方法。目的：研究用含全脂牛奶蛋白或水解酪蛋白肽配方奶粉喂养婴儿的全身免疫反应的发展情况。方法：在一项双盲试验中，10名婴儿服用牛奶配方奶粉，10名婴儿服用酪蛋白水解配方奶粉，直到9个月大。分别在6、9、12个月时采集血样。通过外周血单核细胞对牛奶蛋白（β -乳球蛋白、牛血清白蛋白和α -酪蛋白）的增殖试验来评估细胞反应。用ELISA法检测对相同蛋白的体液反应，检测IgG抗体。结果：用牛奶配方奶粉喂养婴儿可诱导对牛奶蛋白的全身体液和细胞反应。t细胞反应后来下降，支持口服耐受的概念。9个月后暴露于牛奶蛋白导致细胞和体液对这些蛋白质的反应性下降。结论：我们的研究结果支持了人类口腔耐受诱导是一种年龄依赖性现象的观点。
Background: Development of humoral and cellular immune responses to orally administered antigens in human beings is poorly understood, although antigen administration has been suggested as a treatment for hypersensitivity disorders and autoimmune diseases.Objective: The purpose of the study was to investigate the development of systemic immune response in infants fed with formula containing whole cow's milk proteins or hydrolyzed formula containing casein peptides.Methods: In a double-blind trial, 10 infants received cow's milk-based formula, and 10 infants received a casein hydrolysate formula until the age of 9 months. Blood samples were taken at the age of 6, 9, and 12 months. Cellular responses were assessed by proliferation assay of peripheral blood mononuclear cells to cow's milk proteins (beta-lactoglobulin, bovine serum albumin, and alpha-casein). Humoral responses to the same proteins were measured by ELISA for IgG antibodies.Results: Feeding infants with cow's milk-based formula induced systemic humoral and cellular responses to cow's milk proteins. T-cell response later declined, supporting the concept of oral tolerization. Exposure to cow's milk proteins after the age of 9 months resulted in depressed cellular and humoral responsiveness to these proteins.Conclusion: Our results support the view that induction of oral tolerance in human beings is an age-dependent phenomenon.