Identification of cytokeratin antigens in Novikoff ascites hepatoma.
Identification of cytokeratin antigens in Novikoff ascites hepatoma.
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Novikoff 腹水肝癌细胞角蛋白抗原的鉴定。
DOI：
10.1073/pnas.79.10.3138
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发表时间：
1982
影响因子：
11.1
通讯作者：
Hnilica,LS
中科院分区：
文献类型：
--
作者：
Schmidt,WN;Pardue,RL;Tutt,MC;Briggs,RC;Brinkley,BR;Hnilica,LS
对Novikoff腹水肝癌中存在的三种多肽抗原（分子量分别为39，000、49，000和56，000--p39、p49和p56）的进一步鉴定已确定这些蛋白质为细胞角蛋白。二维凝胶电泳显示，这三种蛋白质中的每一种都以几种等电形式存在，都是角蛋白的特征。抗原p39和p56具有与该蛋白质家族相容的氨基酸组成，并且免疫荧光显微镜将抗原定位在指示细胞角蛋白的精细细丝阵列上。使用亲和层析纯化的p39抗原的单特异性抗体用于证明蛋白质p39对Novikoff肝癌细胞具有特异性。这些结果表明细胞角蛋白含有细胞特异性，可用作分化和肿瘤形成的标记物。
Further characterization of three polypeptide antigens present in Novikoff ascites hepatoma (molecular weights 39,000, 49,000, and 56,000--p39, p49, and p56, respectively), has established the identity of these proteins as cytokeratins. Two-dimensional gel electrophoresis revealed that each of the three proteins exists in several isoelectric forms, all characteristic of the keratins. The antigens p39, and p56 had amino acid compositions compatible with this family of proteins, and immunofluorescence microscopy localized the antigens on elaborate filament arrays indicative of the cytokeratins. Antibodies monospecific for the p39 antigen, purified by using affinity chromatography, were used to demonstrate that protein p39 was specific for Novikoff hepatoma cells. These results suggest that the cytokeratins contain cell-specific that can be useful as markers for differentiation and neoplasia.