Utility of Bartonella henselae IgM Western Blot Bands for Serodiagnosis of Cat Scratch Disease
Utility of Bartonella henselae IgM Western Blot Bands for Serodiagnosis of Cat Scratch Disease
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DOI：
10.1128/jcm.01322-17
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发表时间：
2018-01-01
影响因子：
9.4
通讯作者：
Ichihara, Kiyoshi
中科院分区：
文献类型：
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作者：
Otsuyama, Ken-ichiro;Tsuneoka, Hidehiro;Ichihara, Kiyoshi
评价亨氏巴尔通体蛋白印迹(WB)条带在检测抗B。亨氏免疫球蛋白M(IgM)用于猫抓病(CSD)的血清学诊断。用92名临床怀疑患有慢性阻塞性肺病的患者和130名健康人的血清检测了IgM带型。92例患者血清中有49例(53.5%)WB条带阳性。8~10、31~35和70 kDa的三条带与亨氏不动杆菌相关，因为所有阳性血清都至少产生了这三条带中的一条，而健康对照血清中没有一条对这三条带显示出反应性。用常规间接荧光抗体(IFA)法检测92例患者血清中亨氏不动杆菌IgM的阳性率为28.3%(26/92)。这些发现表明，虽然操作繁琐，但IgM-WB试验可以用于CSD的确证诊断，而在对照血清中没有假阳性。提纯特定区带中的蛋白质有助于发展具有更高特异性和灵敏度的IgM酶联免疫吸附试验(IgM-ELISA)。
We evaluated the utility of Western blot (WB) bands of Bartonella henselae in detecting anti-B. henselae immunoglobulin M (IgM) for serodiagnosis of cat scratch disease (CSD). IgM band patterns were examined using sera from 92 patients clinically suspected of having CSD and from 130 healthy individuals. Positive WB bands were observed in 49 (53.5%) of the 92 patient sera. Three bands at 8 to 10, 31 to 35, and 70 kDa were regarded as relevant for B. henselae because all of the positive sera yielded at least one of the three bands, and none of the healthy control sera showed reactivity to any of them. In contrast, the positive rate of the patient sera by conventional indirect fluorescence antibody assay (IFA) for B. henselae IgM was 28.3% (26/92) among the patients. These finding suggest that the IgM-WB assay, although cumbersome to perform, can be used for confirmatory diagnosis of CSD with no false positivity in the control sera. Purification of proteins in the specific bands may contribute to the development of an IgM enzyme-linked immunosorbent assay (IgM-ELISA) with improved specificity and sensitivity.