Osmotic fragility of normal duck erythrocytes as influenced by extracts of Plasmodium lophurae, P. lophurae-infected cells, and plasma.
Osmotic fragility of normal duck erythrocytes as influenced by extracts of Plasmodium lophurae, P. lophurae-infected cells, and plasma.
复制标题
正常鸭红细胞的渗透脆性受斑疟原虫提取物、斑疟原虫感染的细胞和血浆的影响。
DOI：
10.2307/3277307
复制
发表时间：
1969
期刊：
影响因子：
--
通讯作者：
R. Herman
中科院分区：
文献类型：
--
作者：
R. Herman
在体外暴露于失活疟原虫或失活疟原虫感染的鸭红细胞的无细胞提取物后，正常鸭红细胞的渗透脆性显著增加。这种影响的程度可能与寄生虫的发育阶段有关，当大多数寄生虫处于后期分裂形式时，这种影响比当它们主要是年轻的滋养体时更大。这种效应显然是由寄生虫本身产生的，而不是由受感染的红细胞产生的。然而，正常鸭红细胞提取物也具有渗透性诱导脆弱的能力，证明其比幼幼滋养体寄生的红细胞更强。这进一步证明了寄生虫后期分段形式的提取物在诱导渗透脆弱性方面的作用。感染雏鸭的血浆在体外也能使正常鸭红细胞的渗透性增加。疟疾感染中红细胞的渗透脆弱性是否被改变一直是许多研究的主题：Geiman等人（1946），Shen等人（1946），Laser(1948)，以及Devakul和Maegraith（1959）对灵长类疟疾的研究；Danon和Gunders（1962）研究啮齿动物疟疾；以及Fogel等人（1966）对这两种疟疾以及人类和禽类疟疾的研究，都表明渗透脆弱性有所增加。然而，早期的几项研究表明，红细胞在疟疾中的渗透脆弱性仍然没有改变（Foy和Kondi, 1943; Foy, 1948）。本报告是一项研究的结果，研究了在寄生虫生命周期的不同阶段，带红细胞和不带红细胞的lophurae提取物对体外水洗的正常小鸭红细胞的渗透脆弱性的影响。血浆对这类红细胞渗透性脆弱性的影响，以及不含寄生虫的受感染红细胞内可溶性物质的影响，也得到了类似的研究。材料与方法
Osmotic fragility of normal duck erythrocytes was significantly increased after exposure in vitro to cell-free extracts of Plasmodium lophurae or P. lophurae-infected duck red cells. The extent of the effect could be correlated with the developmental stage of the parasite, the effect being greater when most of the parasites were late segmenting forms than when they were mostly young trophozoites. The effect is apparently derived from the parasites themselves rather than from the infected erythrocytes. However, extracts from normal duckling erythrocytes also possess an osmotic fragilityinducing capacity which proved to be greater than that of erythrocytes parasitized by young trophozoites. This demonstrated further the effect of the extract of late segmenting forms of the parasite in inducing osmotic fragility. Plasma from infected ducklings could also produce an increased osmotic fragility of normal duck red cells in vitro. Whether the osmotic fragility of erythrocytes in malarial infections is altered has been the subject of many studies: Geiman et al. (1946), Shen et al. (1946), Laser (1948), and Devakul and Maegraith (1959) with primate malaria; Danon and Gunders (1962) with rodent malaria; and Fogel et al. (1966) with both, plus human and avian malarias, all of which indicate that osmotic fragility is increased. However, that osmotic fragility of red cells in malaria remains unaltered has been suggested by several early studies (Foy and Kondi, 1943; Foy, 1948). The present report is the result of an investigation of the effects of extracts of Plasmodium lophurae with and without red cells at various stages of the parasite's life cycle upon the osmotic fragility of washed, normal duckling red cells in vitro. The effects of plasma upon the osmotic fragility of such erythrocytes have similarly been studied as well as the effect of the soluble material within infected erythrocytes exclusive of the parasites. MATERIALS AND METHODS