NEURONAL-ACTIVITY UP-REGULATES ASTROGLIAL GENE-EXPRESSION
NEURONAL-ACTIVITY UP-REGULATES ASTROGLIAL GENE-EXPRESSION
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DOI：
10.1073/pnas.88.15.6819
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发表时间：
1991-08-01
影响因子：
11.1
通讯作者：
LOTHMAN, E
中科院分区：
文献类型：
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作者：
STEWARD, O;TORRE, ER;LOTHMAN, E
已知神经元基因表达受功能活性调节。这种调节被认为在决定发育神经元的分化和调节成熟神经元的运作中起关键作用。在这里，我们描述了神经元活动的星形胶质细胞基因表达的调节。我们报告说，在大鼠海马神经元的强烈活动（电诱导癫痫发作）导致快速和戏剧性的增加，mRNA的胶质细胞酸性蛋白（GFAP），星形胶质细胞特异性的中间丝蛋白。GFAP mRNA水平增加的网站的刺激，以及在该地区的突触激活所产生的癫痫发作。当癫痫发作被反复诱导了许多天，GFAP mRNA的水平保持慢性升高。然而，GFAP mRNA在刺激停止后的几天内恢复到对照水平。星形胶质细胞基因表达和神经元活性之间的耦合可能是神经元活性调节负责调节脑细胞外微环境的支持细胞功能的机制。
Neuronal gene expression is known to be modulated by functional activity. This modulation is thought to play a key role in determining the differentiation of developing neurons and regulating the operation of mature neurons. Here we describe a regulation of astroglial gene expression by neuronal activity. We report that intense neuronal activity (electrically induced seizures) in rat hippocampus leads to rapid and dramatic increases in mRNA for glial fibrillary acidic protein (GFAP), an astroglia-specific intermediate filament protein. GFAP mRNA levels increased at sites of stimulation as well as in areas that were synaptically activated by the resultant seizures. When seizures were induced repetitively for many days, levels of GFAP mRNA remained chronically elevated. However, GFAP mRNA returned to control levels within a few days after the cessation of stimulation. The coupling between astroglial gene expression and neuronal activity may be a mechanism through which neuronal activity modulates the function of supporting cells that are responsible for regulating the extracellular microenvironment of the brain.