Detection of quantitative trait loci for androstenone, skatole and boar taint in a cross between Large White and Meishan pigs
Detection of quantitative trait loci for androstenone, skatole and boar taint in a cross between Large White and Meishan pigs
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DOI：
10.1111/j.1365-2052.2004.01214.x
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发表时间：
2005-02-01
期刊：
影响因子：
2.4
通讯作者：
Haley, CS
中科院分区：
文献类型：
--
作者：
Lee, GJ;Archibald, AL;Haley, CS
“猪臭”是一种强烈的汗状、尿状难闻的气味，在加热或烹饪一些未阉割的公猪的肉时散发出来。通过对大白猪（LW）与梅山猪（MS）杂交群体F-2代数据的分析，检测与公猪膻味相关性状的数量性状位点（QTL）。对178头85±- 5公斤屠宰的完整公猪的脂肪样本进行了分析，以确定公猪污染的主要来源（雄烯酮、吲哚和粪臭素）。通过训练有素的感官小组（SP）对熟肉中的脂肪和瘦肉样本进行野猪，异常和猪肉风味和气味的评分。对F-2个体及其F-1父母和纯种祖父母进行了覆盖整个基因组的117个标记的扫描。在5%染色体显著性阈值（大约等于全基因组暗示显著性阈值）下，对2、4、6、7和9号染色体上雄烯酮的实验室估计进行QTL检测。然而，只有在6号染色体上有与雄烯酮实验室估计QTL相同或相邻标记间隔的猪风味性状QTL。在第14号染色体上，对吲哚和粪臭素的实验室估计值、粪臭素的SP评分以及瘦肉和脂肪的BF评分进行QTL检测。在所有5种情况下，与LW等位基因相比，MS等位基因总体上增加了估计或得分，但似乎在两个品种中都存在理想和不理想的等位基因。14号染色体上的这个位点在减少公猪粪病发病率方面具有很大的潜力，特别是如果进一步的研究能够确定致病多态性或强相关标记。
'Boar taint' is a strong perspiration-like, urine-like unpleasant odour given off upon heating or cooking of meat from some intact (uncastrated) male pigs. Data from the F-2 generation of a Large White (LW) x Meishan (MS) crossbred population were analysed to detect quantitative trait loci (QTL) for traits associated with boar taint. Fat samples from 178 intact male pigs slaughtered at 85 +/- 5 kg were analysed for the major contributors to boar taint (androstenone, indole and skatole). Fat and lean samples from cooked meat were scored for boar, abnormal and pork flavour and odour by a trained sensory panel (SP). A scan with 117 markers covering the whole genome was performed in the F-2 individuals, together with their F-1 parents and purebred grandparents. At the 5% chromosomal significance threshold (approximately equal to the genome-wide suggestive significance threshold), QTL were detected for the laboratory estimate of androstenone on chromosomes 2, 4, 6, 7 and 9. However, only on chromosome 6 were there QTL for boar flavour (BF) traits in the same or adjacent marker intervals as a QTL for the laboratory estimate of androstenone. On chromosome 14, QTL were detected for the laboratory estimates of indole and skatole, the SP score for skatole and the scores for BF in lean and BF in fat. In all five cases, the MS allele generally increased the estimate or score, compared with the LW allele, but it appeared that desirable and undesirable alleles were present in both breeds. This locus on chromosome 14 has considerable potential for use to reduce the incidence of boar taint, especially if further research can identify the causative polymorphism or strongly associated markers.