Photobiomodulation induces hypotensive effect in spontaneously hypertensive rats
Photobiomodulation induces hypotensive effect in spontaneously hypertensive rats
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DOI：
10.1007/s10103-019-02849-7
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发表时间：
2020-04-01
影响因子：
2.1
通讯作者：
Rodrigues, Gerson J.
中科院分区：
文献类型：
--
作者：
Buzinari, Tereza C.;de Moraes, Thiago F.;Rodrigues, Gerson J.
目的：探讨急性光生物调节对自发性高血压大鼠（SHR）血压的影响。将聚乙烯套管植入雄性SHR的股动脉中。24小时后，基线测量血流动力学参数：收缩压、舒张压、平均动脉压和心率，持续1小时。然后模拟激光照射，记录血流动力学参数1 h。在同一动物中，将激光施加在大鼠腹部的六个不同位置，并且还记录血液动力学参数，直到肿胀效应结束。辐照参数为红色波长（660 nm）;平均光功率100 mW; 56 s/点（6个点）;光斑面积0.0586 cm（2）;辐照度1.71 W/cm（2），产生每点96 J/cm（2）的通量。为了测量血浆NO水平，在记录之前以及在介导的肿胀效应结束之后立即收集血液。光生物调节疗法能够降低69%提交申请的SHR的收缩压，显示收缩压、舒张压和平均动脉压降低。未观察到心率变化。然而，有一个增加血清一氧化氮水平的SHR响应光生物调节。我们的研究结果表明，在660 nm的红色激光急性照射可以引起自发性高血压大鼠的血压升高的影响，可能是通过一种机制，涉及NO的释放，而不改变心率。
To evaluate whether acute photobiomodulation can elicit a hypotensive effect in spontaneously hypertensive rats (SHR). Male SHR were submitted to the implantation of a polyethylene cannula into the femoral artery. After 24 h, baseline measurements of the hemodynamic parameters: systolic, diastolic, and mean arterial pressure, and heart rate were accomplished for 1 h. Afterwards, laser application was simulated, and the hemodynamic parameters were recorded for 1 h. In the same animal, the laser was applied at six different positions of the rat's abdomen, and the hemodynamic parameters were also recorded until the end of the hypotensive effect. The irradiation parameters were red wavelength (660 nm); average optical power of 100 mW; 56 s per point (six points); spot area of 0.0586 cm(2); and irradiance of 1.71 W/cm(2) yielding to a fluency of 96 J/cm(2) per point. For measuring plasma NO levels, blood was collected before the recording, as well as immediately after the end of the mediated hypotensive effect. Photobiomodulation therapy was able to reduce the systolic arterial pressure in 69% of the SHR submitted to the application, displaying a decrease in systolic, diastolic, and mean arterial pressure. No change in heart rate was observed. Nevertheless, there was an increase in serum nitric oxide levels in the SHR responsive to photobiomodulation. Our results suggest that acute irradiation with a red laser at 660 nm can elicit a hypotensive effect in SHR, probably by a mechanism involving the release of NO, without changing the heart rate.