Nuphar lutea: In vitro anti-leishmanial activity against Leishmania major promastigotes and amastigotes
Nuphar lutea: In vitro anti-leishmanial activity against Leishmania major promastigotes and amastigotes
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DOI：
10.1016/j.phymed.2009.01.011
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发表时间：
2009-08-01
期刊：
影响因子：
7.9
通讯作者：
Golan-Goldhirsh, A.
中科院分区：
文献类型：
--
作者：
El-On, J.;Ozer, L.;Golan-Goldhirsh, A.
几种抗利什曼病药物的选择是植物来源。许多可用的抗该病药物是有毒的，在某些情况下，寄生虫产生了抗药性。本研究的目的：测定黄花豆植物提取物对硕大利什曼原虫的体外杀利什曼原虫活性。主要的游离前鞭毛体和胞内无鞭毛体，通过显微镜检查和酶XTT测定进行评价。lutea对利什曼原虫前鞭毛体和利什曼原虫均高度有效。无鞭毛体（IC 50 = 2 ± 0.12 μ g/ml; ID 50 = 0.65 ± 0.023 μ g/ml; LD 50 = 2.1 ± 0.096 μ g/ml，STI = 3.23）。1.25 μ g/ml的提取物在处理3天内完全消除了细胞内寄生虫。此外，协同抗利什曼原虫活性被证明与N。与抗利什曼原虫药物巴龙霉素联合使用。部分纯化的N.结果表明，黄茶叶中的活性成分是一种热稳定的生物碱，不带电荷，耐煮沸，耐甲醇、二氯甲烷和二甲苯处理。叶黄素可能是抗利什曼原虫化合物的潜在来源。(C)2009年由Elsevier GmbH出版。
Several anti-leishmanial drugs of choice are of plant origin. Many of the available drugs against the disease are toxic and in certain cases parasite drug resistance is developed. The development of new compounds is urgently required.Aims of the study: To determine the leishmanicidal activity of the Nuphar lutea plant extract against Leishmania major in vitro.Materials and methods: The leishmanicidal activity of methanolic plant extract against L. major free living promastigotes and intracellular amastigotes was evaluated, using microscopic examinations and the enzymatic XTT assay.Results: Methanolic extract of N. lutea was highly effective against both Leishmania promastigotes and L. amastigotes (IC50 = 2 +/- 0.12 mu g/ml; ID50 = 0.65 +/- 0.023 mu g/ml; LD50 = 2.1 +/- 0.096 mu g/ml, STI = 3.23). The extract at 1.25 mu g/ml totally eliminated the intracellular parasites within 3 days of treatment. Also, a synergistic anti-leishmanial activity was demonstrated with N. lutea extract combined with the anti-leishmanial drug - paromomycin. The partially purified N. lutea active component was found to be a thermo-stable alkaloid(s) with no electrical charge and is resistant to boiling and to methanol, dichloromethane and xylene treatment.Conclusions: The present study suggests that N. lutea might be a potential source of anti-leishmanial compounds. (C) 2009 Published by Elsevier GmbH.