Quantitative and spatial information on the composition of chimaeric fetal mouse eyes from single histological sections
Quantitative and spatial information on the composition of chimaeric fetal mouse eyes from single histological sections
复制标题
单个组织切片中嵌合胎儿小鼠眼睛组成的定量和空间信息
DOI：
10.1046/j.1440-169x.1997.t01-2-00006.x
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发表时间：
1997
期刊：
影响因子：
4.6
通讯作者：
M. Keighren
中科院分区：
文献类型：
--
作者：
J. West;B. Hodson;M. Keighren
由两种基因型组成的嵌合体组织中细胞的空间分布提供了对发育和生长过程中细胞混合程度的了解。然而，斑块大小的直接测量通常是没有意义的，因为，当一个基因型的比例很高，一个单一的补丁可能包括几个相邻的相干克隆相似的基因型（克隆聚集）。两个以前使用的方法比较补丁长度进行了评估，以克服这个问题。校正后的平均斑块长度（校正随机克隆聚集的预测影响）是一个更有用的汇总统计比中位斑块长度的次要基因型，因为它的使用并不限于严重不平衡的嵌合体，但其有效性受到质疑。这两种方法给出了几乎相同的数字摘要补丁大小在视网膜色素上皮细胞的胎儿嵌合体，从而验证了使用校正的平均补丁长度为该组织。本研究还表明，校正的斑块长度不受TgN（Hbb‐b1）83 Clo转基因半合子细胞存在的影响，并且单个组织学切片中色素细胞的比例代表嵌合体胎儿的总体组成。
The spatial distribution of cells in chimaeric tissues, composed of two genotypes, provides insights into the extent of cell mixing during development and growth. However, direct measurement of patch sizes is not usually meaningful because, when the proportion of one genotype is high, a single patch may encompass several adjacent coherent clones of like genotype (clone aggregation). Two previously used methods of comparing patch lengths were evaluated to overcome this problem. The corrected mean patch length (corrected for the predicted effects of random clone aggregation) is a more useful summary statistic than the median patch length of the minor genotype, because its use is not restricted to grossly unbalanced chimaeras, but its validity has been questioned. The two methods gave almost identical numerical summaries of patch sizes in the retinal pigment epithelium of fetal chimaeras, thereby validating the use of the corrected mean patch length for this tissue. The present study also showed that the corrected patch length was unaffected by the presence of cells hemizygous for the TgN(Hbb‐b1)83Clo transgene and that the proportion of pigmented cells in a single histological section was representative of the overall composition of the chimaeric fetus.
影响因子：
2.9
作者：
LO, CW;COULLING, M;KIRBY, C
通讯作者：
KIRBY, C
马赛克图案的分形几何表明肝脏再生是一个自相似的过程。
影响因子：
2.7
作者：
Ng,YK;Iannaccone,PM
通讯作者：
Iannaccone,PM
大鼠嵌合体的分形几何表明，重复的细胞分裂程序可能会产生肝实质。
影响因子：
2.7
作者：
Khokha,MK;Landini,G;Iannaccone,PM
通讯作者：
Iannaccone,PM