Early activation of mucosal dendritic cells and macrophages in acute Campylobacter colitis and cholera:: An in vivo study
Early activation of mucosal dendritic cells and macrophages in acute Campylobacter colitis and cholera:: An in vivo study
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DOI：
10.1111/j.1440-1746.2008.05325.x
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发表时间：
2008-05-01
影响因子：
4.1
通讯作者：
Balasubramanian, Kunnissery A.
中科院分区：
文献类型：
--
作者：
Pulimood, Anna B.;Ramakrishna, Balakrishna S.;Balasubramanian, Kunnissery A.
背景与目的：巨噬细胞和树突状细胞是两种关系密切的单核吞噬细胞。关于它们在人类急性肠道细菌感染中的体内作用知之甚少。方法：对急性弯曲杆菌性结肠炎（n = 5）、志贺氏菌病（n = 5）和霍乱（n = 10）患者直肠粘膜活检组织中的所有粘膜单核吞噬细胞进行超微结构评价，并与对照组（n = 5）进行比较。直肠粘膜浅层单核细胞吞噬细胞在弯曲杆菌性结肠炎和霍乱中的激活的超微结构特征的患病率高于对照组。志贺氏菌病患者单核细胞活化特征的发生率较低。具有活化树突状细胞的超微结构形态的细胞在5名弯曲杆菌结肠炎患者中的2名中占所有单核吞噬细胞的41%和45%，在10名霍乱患者中的4名中占4-22%。弯曲杆菌结肠炎患者的存在与显着的表面上皮损伤和突出的急性炎症变化mucosa.Conclusions：这是第一次超微结构的研究，显示激活的巨噬细胞和树突状细胞在体内急性弯曲杆菌结肠炎和霍乱。树突状细胞活化发生在这些感染的临床过程的早期。表面上皮损伤可能在树突状细胞的活化中起作用。
Background and Aim: Macrophages and dendritic cells are closely related mononuclear phagocytic cells. Little is known about their in vivo role in acute intestinal bacterial infections in humans. We undertook to evaluate these cells in rectal mucosal biopsies of patients with acute colitis.Methods: All mucosal mononuclear phagocytic cells in rectal biopsies of patients with acute Campylobacter colitis (n = 5), shigellosis ( n = 5), and cholera ( n = 10) were evaluated ultrastructurally and compared with those in controls ( n = 5).Results: Mononuclear phagocytic cells in the superficial rectal mucosa showed a higher prevalence of ultrastructural features of activation in Campylobacter colitis and cholera than in controls. A lower prevalence of features of activation with increased monocytes was seen in shigellosis. Cells with the ultrastructural morphology of activated dendritic cells constituted 41% and 45% of all mononuclear phagocytic cells in two of five patients with Campylobacter colitis and 4-22% of cells in four of 10 patients with cholera. Their presence in patients with Campylobacter colitis was associated with significant surface epithelial damage and prominent acute inflammatory changes in the mucosa.Conclusions: This is the first ultrastructural study to show activated macrophages and dendritic cells in vivo in acute Campylobacter colitis and cholera. Dendritic cell activation occurred early in the clinical course of these infections. Surface epithelial damage may play a role in the activation of dendritic cells.