Free Fatty Acids Activate Renin-Angiotensin System in 3T3-L1 Adipocytes through Nuclear Factor-kappa B Pathway.
Free Fatty Acids Activate Renin-Angiotensin System in 3T3-L1 Adipocytes through Nuclear Factor-kappa B Pathway.
复制标题
游离脂肪酸通过核因子-κ B 途径激活 3T3-L1 脂肪细胞中的肾素-血管紧张素系统
DOI：
10.1155/2016/1587594
复制
发表时间：
2016
影响因子：
4.3
通讯作者：
Chen H
中科院分区：
文献类型：
--
作者：
Sun J;Luo J;Ruan Y;Xiu L;Fang B;Zhang H;Wang M;Chen H
脂肪组织中局部肾素-血管紧张素系统（RAS）的活性与肥胖相关疾病密切相关。然而，RAS在脂肪组织中激活的机制仍然未知。本研究发现，游离脂肪酸棕榈酸（palmitic acid，PA）可诱导3 T3-L1脂肪细胞RAS活性。在胎球蛋白A（FetA）存在下，PA上调3 T3-L1脂肪细胞血管紧张素原（AGT）和血管紧张素1型受体（AT 1 R）的表达，并刺激血管紧张素II（ANG II）的分泌。此外，当我们使用TAK 242阻断Toll样受体4（TLR 4）信号通路或使用BAY 117082阻断NF-κB信号通路时，3 T3-L1脂肪细胞中RAS的激活被阻断。总之，我们的研究结果已经确定了关键的分子机制连接PA/TLR 4/NF-κB信号通路的活动，局部的肾素-血管紧张素系统在脂肪组织。
The activity of a local renin-angiotensin system (RAS) in the adipose tissue is closely associated with obesity-related diseases. However, the mechanism of RAS activation in adipose tissue is still unknown. In the current study, we found that palmitic acid (PA), one kind of free fatty acid, induced the activity of RAS in 3T3-L1 adipocytes. In the presence of fetuin A (Fet A), PA upregulated the expression of angiotensinogen (AGT) and angiotensin type 1 receptor (AT1R) and stimulated the secretion of angiotensin II (ANG II) in 3T3-L1 adipocytes. Moreover, the activation of RAS in 3T3-L1 adipocytes was blocked when we blocked Toll-like receptor 4 (TLR4) signaling pathway using TAK242 or NF-κB signaling pathway using BAY117082. Together, our results have identified critical molecular mechanisms linking PA/TLR4/NF-κB signaling pathway to the activity of the local renin-angiotensin system in adipose tissue.