SNOM Imaging of a Crypt-Like Feature in Adenocarcinoma Associated with Barrett's Oesophagus
SNOM Imaging of a Crypt-Like Feature in Adenocarcinoma Associated with Barrett's Oesophagus
复制标题
DOI：
10.1002/pssb.201700518
复制
发表时间：
2018-04-01
影响因子：
1.6
通讯作者：
Siggel-King, Michele R. F.
中科院分区：
文献类型：
--
作者：
Craig, Timothy;Smith, Andrew D.;Siggel-King, Michele R. F.
发展更准确和灵敏的诊断技术是提高癌症存活率的关键因素。红外孔径纤维扫描近场光学显微镜（IR-SNOM）的技术，连同辐射的红外自由电子激光器（IR-FEL）的ALICE在达雷斯伯里实验室（英国），已被用来获得红外图像的隐窝样功能和周围组织的组织，该组织取自患者的食管腺癌和历史的巴雷特食管。我们已经表明，DNA信号增强相对于其他的贡献在该地区的隐窝，和糖蛋白信号显示一个不太明显的增加在该地区的隐窝。发现酰胺II信号与DNA和糖蛋白谱反相关。发现酰胺II信号的吸光度对于三种不同类型的癌组织是不同的。高分辨率红外图像的隐窝揭示了额外的结构，将无法解决衍射限制技术。
The development of more accurate and sensitive diagnostic techniques is a key factor in efforts to improve cancer survival rates. The technique of infrared aperture fibre scanning near-field optical microscopy (IR-SNOM), together with radiation from the infrared free-electron laser (IR-FEL) on ALICE at Daresbury Laboratory (UK), has been used to obtain IR images of a crypt-like feature and the surrounding tissue; the tissue was taken from a patient with oesophageal adenocarcinoma and with a history of Barrett's oesophagus. We have shown that the DNA signal is enhanced relative to other contributions in the region of the crypt, and the glycoprotein signal shows a less pronounced increase in the region of the crypt. The Amide II signal is found to be anti-correlated with the DNA and glycoprotein profiles. The absorbance of the Amide II signal is found to differ for three different types of cancer tissue. High-resolution IR images of the crypt reveal additional structure that would not be resolved in diffraction-limited techniques.