CRYOBIOLOGY OF INVITRO-DERIVED BOVINE EMBRYOS
CRYOBIOLOGY OF INVITRO-DERIVED BOVINE EMBRYOS
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DOI：
10.1016/0093-691x(93)90025-z
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发表时间：
1993-01-01
期刊：
影响因子：
2.8
通讯作者：
LOSKUTOFF, NM
中科院分区：
文献类型：
--
作者：
LEIBO, SP;LOSKUTOFF, NM
通过体外成熟、受精和培养方法从卵巢卵母细胞生产牛胚胎的能力在过去几年中发展非常迅速。这些体外衍生胚胎的有效实验和实际应用需要将它们冷冻保存。直到最近，体外胚胎被认为比体内胚胎对冷冻更敏感。最近的观察开始揭示，体外冷冻保存胚胎的高存活率需要成熟和培养技术的改进，而不是简单地改变冷冻保存方法。哺乳动物胚胎学的这两个方面的融合正在取得成功;由于移植了冷冻保存的体外衍生胚胎，已经产生了妊娠和小牛。
The capability to produce bovine embryos from ovarian oocytes by in vitro methods of maturation, fertilization and culture has progressed very rapidly during the past few years. Efficient experimental and practical use of these in vitro-derived embryos requires that they be cryopreserved. Until recently, in vitro embryos were considered to be much more sensitive to freezing than in vivo embryos. Recent observations are beginning to reveal that high survival of cryopreserved in vitro embryos requires improvements in the techniques of maturation and culture, rather than simply changes in cryopreservation methods. The merging of these two aspects of mammalian embryology is yielding success; pregnancies have been produced and calves have been born as a result of the transfer of cryopreserved in vitro-derived embryos.