Polyamine sensing during antizyme mRNA programmed frameshifting
Polyamine sensing during antizyme mRNA programmed frameshifting
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DOI：
10.1016/j.bbrc.2005.10.115
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发表时间：
2005-12-23
影响因子：
3.1
通讯作者：
Atkins, JF
中科院分区：
文献类型：
--
作者：
Petros, LM;Howard, MT;Atkins, JF
从酵母到哺乳动物，细胞多胺水平的关键调节因子是蛋白质抗酶。该抗酶基因由两个重叠的阅读框组成，ORF2位于相对于ORFI的+1框。一个程序化的+1核糖体移码发生在ORFI的最后一个密码子上，并导致全长抗酶蛋白的产生。帧移的效率与多胺的浓度成正比，从而创建了一个控制多胺水平的自调节回路。用于移帧的mRNA重编码信号包括移位位点的一个元件5‘类假结3’。目前的研究表明，ORFI停止密码子类型的5‘元件对多胺传感至关重要，而3’假结以独立于多胺的方式刺激帧移。我们还证明，在正常表达GagPol前体蛋白所需的MuLV重定义位点，需要多胺来刺激停止密码子读取。(c) 2005爱思唯尔公司版权所有。
A key regulator of cellular polyamine levels from yeasts to mammals is the protein antizyme. The antizyme gene consists of two overlapping reading frames with ORF2 in the +1 frame relative to ORFI. A programmed +1 ribosomal frameshift occurs at the last codon of ORFI and results in the production of full-length antizyme, protein. The efficiency of frameshifting is proportional to the concentration of polyamines, thus creating kin autoregulatory circuit for controlling polyamine levels. The mRNA recoding signals for frameshifting include an element 5' kind a pseudodoknot 3' of the shift site. The present work illustrates that the ORFI stop codon kind the 5' element are critical for polyamine sensing, whereas the 3' pseudoknot acts to stimulate frameshifting in a polyamine independent manner. We also demonstrate that polyamines are required to Stimulate stop codon readthrough at the MuLV redefinition site required for normal expression of the GagPol precursor protein. (c) 2005 Elsevier Inc. All rights reserved.