Three-dimensional microCT imaging of mouse development from early post-implantation to early postnatal stages
Three-dimensional microCT imaging of mouse development from early post-implantation to early postnatal stages
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DOI：
10.1016/j.ydbio.2016.09.011
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发表时间：
2016-11-15
影响因子：
2.7
通讯作者：
Dickinson, Mary E.
中科院分区：
文献类型：
--
作者：
Hsu, Chih-Wei;Wong, Leeyean;Dickinson, Mary E.
在这项工作中，我们报告了使用碘对比微ct对小鼠胚胎和新生儿在胚胎第8.5天至出生后第3天进行高通量3D形态学分析，高空间分辨率高达3 μ m/体素。我们发现，在不干扰胚胎的情况下，早期阶段的小鼠胚胎可以在卵黄囊或蜕膜等胚胎外组织内成像。这种方法能够对胚胎转向、尿囊发育、卵黄血管重塑、卵黄囊和早期胎盘发育进行完整、不受干扰的分析，从而为突变系的早期胚胎致死性提供了更多的见解。此外，这些方法价格低廉，学习简单，不需要大量的处理时间，使它们成为高通量分析具有胚胎和出生后早期致死率的小鼠突变体的理想方法。
In this work, we report the use of iodine-contrast microCT to perform high-throughput 3D morphological analysis of mouse embryos and neonates between embryonic day 8.5 to postnatal day 3, with high spatial resolution up to 3 mu m/voxel. We show that mouse embryos at early stages can be imaged either within extra embryonic tissues such as the yolk sac or the decidua without physically disturbing the embryos. This method enables a full, undisturbed analysis of embryo turning, allantois development, vitelline vessels remodeling, yolk sac and early placenta development, which provides increased insights into early embryonic lethality in mutant lines. Moreover, these methods are inexpensive, simple to learn and do not require substantial processing time, making them ideal for high throughput analysis of mouse mutants with embryonic and early postnatal lethality.