The Tribolium chitin synthase genes TcCHS1 and TcCHS2 are specialized for synthesis of epidermal cuticle and midgut peritrophic matrix
The Tribolium chitin synthase genes TcCHS1 and TcCHS2 are specialized for synthesis of epidermal cuticle and midgut peritrophic matrix
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DOI：
10.1111/j.1365-2583.2005.00576.x
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发表时间：
2005-10-01
影响因子：
2.6
通讯作者：
Beeman, RW
中科院分区：
文献类型：
--
作者：
Arakane, Y;Muthukrishnan, S;Beeman, RW
两个几丁质合成酶基因，TcCHS 1和TcCHS 2，在红粉甲虫，赤拟谷盗的功能分析，揭示了每个基因的独特和互补的作用。TcCHS 1特异性RNA干扰（RNAi）破坏了所有三种类型的蜕皮（幼虫-幼虫，幼虫-蛹和蛹-成虫），并大大降低了全身几丁质含量。外显子特异性RNAi显示TcCHS 1的剪接变体8a对于幼虫-蛹和蛹-成虫蜕皮都是必需的，而剪接变体8b仅对于后者是必需的。TcCHS 2特异性RNAi对变态或全身几丁质含量没有影响。然而，RNAi介导的下调TcCHS 2，而不是TcCHS 1，导致停止喂养，幼虫大小急剧缩小，中肠几丁质含量减少。
Functional analysis of the two chitin synthase genes, TcCHS1 and TcCHS2, in the red flour beetle, Tribolium castaneum, revealed unique and complementary roles for each gene. TcCHS1-specific RNA interference (RNAi) disrupted all three types of moult (larval-larval, larval-pupal and pupal-adult) and greatly reduced whole-body chitin content. Exon-specific RNAi showed that splice variant 8a of TcCHS1 was required for both the larval-pupal and pupal-adult moults, whereas splice variant 8b was required only for the latter. TcCHS2-specific RNAi had no effect on metamorphosis or on total body chitin content. However, RNAi-mediated down-regulation of TcCHS2, but not TcCHS1, led to cessation of feeding, a dramatic shrinkage in larval size and reduced chitin content in the midgut.