Alveolar bone loss induced by the orthodontic tooth movement under hypofunctional conditions in rats.
Alveolar bone loss induced by the orthodontic tooth movement under hypofunctional conditions in rats.
复制标题
大鼠功能低下条件下正畸牙齿移动引起的牙槽骨丢失。
DOI：
10.1016/j.odw.2013.07.002
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发表时间：
2013
影响因子：
0.4
通讯作者：
Takashi Ono
中科院分区：
文献类型：
--
作者：
Chisa Shitano;Otto Baba;Sawa Kaneko;Jun Hosomichi;Yasuhiro Shimizu;Naoki Shibutani,; Risa Usumi-Fujita;Yoshiro Takano;Takashi Ono
目的建立大鼠正畸模型，探讨咬合功能障碍对正畸牙齿运动的影响及其与牙槽骨结构和质量的关系。材料与方法将12周龄雄性sd - dawley大鼠分为4组，每组8只：正常咬合(N)组、正常咬合伴牙动(M)组、咬合功能减退(H)组、咬合功能减退伴牙动（HM）组。H组和HM组采用光固化复合树脂将前咬合板和金属帽附着于上颌门牙和下颌门牙上，诱导牙合功能减退。M组和HM组在上颌第一磨牙（M1）颊面用镍钛合金丝沿腭方向施加正畸力。采用荧光骨标记技术对M1s周围牙槽骨进行显微ct成像和组织形态学分析。结果HM组与M组相比，M1的平滑肌运动速度明显加快，倾斜增强。Micro-CT分析显示，实验组中HM组骨体积分数、骨矿物质密度和根间骨小梁厚度均明显降低。与N组相比，H组和M组根间骨荧光标记线数量减少。HM组可见少量不连续的不规则虚线样标记。结论咬合功能障碍加速了正畸牙各自的移动，同时导致牙槽骨周围严重的骨质流失。
PurposeTo examine the effect of occlusal hypofunctional conditions on orthodontic tooth movement and its relation to the structure and quality of alveolar bone using the rat model.Materials and methodsTwelve-week-old male Sprague-Dawley rats were divided into 4 groups of 8 animals each: normal occlusion (N) group, normal occlusion with tooth movement (M) group, occlusal hypofunction (H) group, and occlusal hypofunction with tooth movement (HM) group. In H and HM groups, the anterior bite plate and metal cap were attached to the maxillary and mandibular incisors using a light-curing composite resin to induce the occlusal hypofunctional condition. In M and HM groups, an orthodontic force was applied in a palatal direction to the buccal surface of the maxillary first molar (M1) using a nickel–titanium alloy wire. Micro-CT imaging and histomorphometric analysis using fluorescent bone labeling of the alveolar bone surrounding the M1s were performed in each group.ResultsTooth movement of M1 in HM group, was rather accelerated with enhanced tipping than in M group. Micro-CT analysis revealed significant decrease in bone volume fraction, bone mineral density and trabecular thickness of the interradicular bone in HM group among the experimental groups. The fluorescent labeling lines in the interradicular bone were decreased in number in H and M groups compared with N group. A few discontinuous irregular dotted lines-like labeling was observed in HM group.ConclusionThe occlusal hypofunctional condition accelerates orthodontic tooth movement of the respective teeth, while it results in severe bone loss in the surrounding alveolar bone.