Improved method for measuring C1-r-C1-s-(C1 inh)2 complexes by an enzyme-linked immunosorbent assay.
Improved method for measuring C1-r-C1-s-(C1 inh)2 complexes by an enzyme-linked immunosorbent assay.
复制标题
通过酶联免疫吸附测定测量 C1-r-C1-s-(C1 inh)2 复合物的改进方法。
DOI：
10.1002/jcla.1860090309
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发表时间：
1995
影响因子：
2.7
通讯作者：
Zuraw,BL
中科院分区：
文献类型：
--
作者：
Mathews,KP;Herschbach,JH;Chambers,SL;Zuraw,BL
采用酶联免疫吸附试验（ELISA）测定血清或血浆中的C1rC1s - (C1 inh)2复合物是一种相对方便和敏感的评估C1活化的方法。然而，在低血清或血浆稀释度下，未激活的C1 q (r‐s)2的干扰导致估计值随血清或血浆稀释度的不同而有很大差异。有人提出用6%聚乙二醇沉淀干扰C1q (r‐s)2来解决这个问题，但这里的研究表明，这一过程也会沉淀或共沉淀一些C1rC1s - C1rC1s - (C1 inh)2配合物。在不需要去除C1q (r - s)2的情况下，通过测试高等离子体稀释度，在没有PEG沉淀的情况下获得了令人满意的结果。研究了在高稀释度条件下定量测定这些配合物的最佳条件。正常EDTA血浆C1rC1s‐C1rC1s‐（C1 inh）2复合体测量平均值为36.6±7.0 （S.D.）。ELISA单位，95%可信区间为19.5-47.6u。除了为C1激活提供一种灵敏的检测方法外，测量C1rC1s - C1rC1s - (C1 inh)2复合物可能有助于阐明各种条件下C1 inh缺乏的病理生理机制。©1995 wiley‐Liss, inc。
Measurement of C1rC1s‐(C1 inh)2complexes in serum or plasma by enzyme‐linked immunosorbent assay (ELISA) has been proposed as a relatively convenient and sensitive means for assessing C1 activation. However, interference by unactivated C1 q (r‐s)2at low serum or plasma dilutions has resulted in estimates that vary widely with the degree of serum or plasma dilution. Precipitating the interfering C1q (r‐s)2with 6% polyethylene glycol has been proposed to resolve this problem, but here it is shown that this procedure also precipitates or coprecipitates some of the C1rC1s‐C1rC1s‐(C1 inh)2complexes. Satisfactory results have been achieved without PEG precipitation by testing high plasma dilutions under conditions where there is a sufficient excess of anti‐C1s coating the microtitration plate wells that removal of C1q (r‐s)2is not necessary. Optimizing conditions for quantitating these complexes at high dilution have been investigated. The mean normal EDTA plasma C1rC1s‐C1rC1s‐(C1 inh)2complex measurement was 36.6±7.0 (S.D.) ELISA units with a 95% confidence interval of 19.5–47.6u. Besides providing a sensitive assay for C1 activation, measuring C1rC1s‐C1rC1s‐(C1 inh)2complexes may help to clarify the pathophysiologic mechanisms resulting from C1 inh deficiency under various conditions.©1995 wiley‐Liss, inc.