Mode of binding of ionic materials to casein micelles
Mode of binding of ionic materials to casein micelles
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DOI：
10.1017/s0022029900022172
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发表时间：
1982-02
影响因子：
2.1
通讯作者：
M. L. Green
中科院分区：
文献类型：
--
作者：
M. L. Green
总结测定了吸附到酪蛋白胶束上并加速牛奶凝固的材料的比例，并在用牛奶透析液稀释胶束悬浮液时保持结合。这高于预期的假设，即平衡发生在溶液和胶束上的许多等效结合位点之间。这表明结合涉及疏水相互作用和多点连接到带电基团的任一种或两种。在胶束中的添加剂结合位点的位置进行了研究，使用模型酪蛋白酸钠和羟基磷灰石在pH 6·6。加速凝血的材料与任一或两种模型结合。那些对牛奶凝固的影响最大的可能主要结合到酪蛋白，而不是酪蛋白胶束的胶体磷酸钙部分。
SUMMARY The proportion of materials which adsorb to casein micelles and accelerate milk coagulation, and remain bound on dilution of the micelle suspension with milk dialysate was determined. This was higher than expected from the postulate that equilibration took place between the solution and many equivalent binding sites on the micelle. This suggests that binding involved either or both hydrophobic interactions and multipoint attachment to charged groups. The location of the additive binding sites in the micelle was investigated using models Na caseinate and hydroxyapatite at pH 6·6. Materials which accelerated coagulation bound to either or both models. Those with the greatest effect on milk coagulation probably bound primarily to the casein rather than to the colloidal calcium phosphate moiety of casein micelles.