Roles of the telencephalic cells and their chondroitin sulfate proteoglycans in delimiting an anterior border of the retinal pathway
Roles of the telencephalic cells and their chondroitin sulfate proteoglycans in delimiting an anterior border of the retinal pathway
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DOI：
10.1523/jneurosci.21-23-09304.2001
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发表时间：
2001-12-01
影响因子：
5.3
通讯作者：
Kawabata, I
中科院分区：
文献类型：
--
作者：
Ichijo, H;Kawabata, I
视网膜神经节细胞的轴突在间脑端脑边界上延伸至顶盖；然而，它们不会从前面侵入端脑。为了研究阻止视网膜轴突进入端脑区域的机制，在体外检查了端脑细胞对视网膜轴突生长的影响；视网膜的生长被来自端脑的细胞底物选择性地抑制。此外，在端脑膜和来自端脑的外周膜分子部分中发现了选择性抑制的负责因素。由于抑制作用被软骨素酶 ABC 破坏，而不是被热破坏，因此这种抑制归因于附着在端脑细胞膜上的硫酸软骨素蛋白聚糖 (CSPG) 的碳水化合物链。为了了解端脑CSPG在体内视网膜寻路中的功能，通过脑室内注射软骨素酶ABC从胚胎脑中去除它们的碳水化合物链[硫酸软骨素糖胺聚糖（CS-GAG）]； CS-GAG 的去除导致视束前部扩大。结果表明，端脑细胞用它们的 CSPG 界定视束的前缘，并防止视网膜轴突异常进入前部区域。
The axons of the retinal ganglion cells run on the diencephalotelencephalic boundary on their way to the tectum; however, they do not invade the telencephalon anteriorly. To investigate the mechanisms that prevent the retinal axons from entering the telencephalic territory, the effects of the telencephalic cells were examined on the outgrowth of the retinal axons in vitro; the retinal outgrowth was selectively inhibited by the cellular substrate derived from the telencephalon. The responsible factor for the selective inhibition was, furthermore, found in the telencephalic membranes and the fraction of peripheral membrane molecules from the telencephalon. Because the inhibitory effect was destroyed by chondroitinase ABC but not by heat, this inhibition was attributable to the carbohydrate chains of chondroitin sulfate proteoglycans (CSPGs) adhering to the membranes of the telencephalic cells. To understand the function of the telencephalic CSPGs on the retinal pathfinding in vivo, their carbohydrate chains [chondroitin sulfate glycosaminoglycan (CS-GAG)] were removed from the embryonic brains by intraventricular injection of chondroitinase ABC; the removal of CS-GAG resulted in an anterior enlargement of the optic tract. The results indicate that the telencephalic cells delimit the anterior border of the optic tract with their CSPGs and prevent the retinal axons from aberrantly entering the anterior territory.