Genetic manipulation of Clostridium difficile.
Genetic manipulation of Clostridium difficile.
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DOI：
10.1002/9780471729259.mc09a02s20
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发表时间：
2011-02-01
影响因子：
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通讯作者：
Sorg, Joseph A
中科院分区：
文献类型：
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作者：
Bouillaut, Laurent;McBride, Shonna M;Sorg, Joseph A
艰难梭菌是一种革兰氏阳性、孢子形成、厌氧的肠道细菌，是结肠炎相关性结肠炎的最常见原因。多年来，这种生物体被认为是遗传上难以处理的，但在过去的10年里，已经开发或采用了多种方法来遗传操纵C。很难本单元描述了用于这种生物体的遗传修饰的分子技术，包括基因破坏，互补，质粒引入和整合以及跨物种接合的方法。
Clostridium difficile is a Gram-positive, spore forming, anaerobic, intestinal bacterium and is the most common cause of antibiotic-associated colitis. For many years this organism was considered genetically intractable, but in the past 10 years, multiple methods have been developed or adapted for genetic manipulation of C. difficile. This unit describes the molecular techniques used for genetic modification of this organism, including methods for gene disruption, complementation, plasmid introduction and integration, and cross-species conjugations.