Picornavirus infections in children diagnosed by RT-PCR during longitudinal surveillance with weekly sampling: Association with symptomatic illness and effect of season
Picornavirus infections in children diagnosed by RT-PCR during longitudinal surveillance with weekly sampling: Association with symptomatic illness and effect of season
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DOI：
10.1002/jmv.20588
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发表时间：
2006-05-01
影响因子：
12.7
通讯作者：
Hendley, JO
中科院分区：
文献类型：
--
作者：
Winther, B;Hayden, FG;Hendley, JO
RT-PCR对鼻病毒检测比细胞培养更敏感，但在横断面研究中，健康对照经常呈鼻病毒（或小核糖核酸病毒）阳性。对15名健康儿童进行一年至少三个季节的随访，每周采集鼻/鼻咽分泌物，进行小核糖核酸病毒RT-PCR检测，并每天记录呼吸道症状。一份小核糖核酸病毒阳性样本被诊断为感染；连续阳性的每周样本构成一次感染。如果在发病前7天至发病后21天检测到RNA，则诊断为小核糖核酸疾病。每周740份样本中155份（21%）呈小核糖核酸病毒阳性并与疾病相关；37例（5%）阳性与疾病无关（P=0.001）。192个阳性样本发生在121个感染病例中，74例为单一阳性，47例在两个或多个连续样本中“连续”出现阳性。47例中有45例（96%）连续2或3例阳性。235个月期间185例报告疾病中有96例（52%）呈小核糖核酸病毒阳性（0.4例/个月）；无症状感染者25例（0.11例/月）。秋季感染率最高（0.66/个月）；冬季发病率（0.44/儿月）与春季（0.5）和夏季（0.43）相似。小核糖核酸病毒感染在健康儿童中很常见（0.51个/个月），是间歇性的，通常与短暂的疾病有关；五分之一的感染者无症状。秋季感染率最高；冬季的感染率与春季和夏季相同。(c) 2006 Wiley-Liss, Inc。
RT-PCR is more sensitive for rhinovirus detection than cell culture, but healthy controls are frequently rhinovirus (or picornavirus) positive in cross-sectional studies. Fifteen healthy children were followed over at least three seasons of the year with weekly sampling of nasal/nasopharyngeal secretion for RT-PCR testing for picornavirus and daily recording of respiratory symptoms. One sample positive for picornavirus was diagnosed as an infection;.consecutive positive weekly samples constituted a single infection. Picornavirus illness was diagnosed if RNA was detected 7 days prior through 21 days after onset. One hundred fifty-five (21%) of 740 weekly samples were picornavirus positive and associated with illness; 37(5%) positives were not associated with illness (P=0.001). The 192 positive samples occurred in 121 infections, 74 with a single positive and 47 with "runs" of positives in two or more consecutive samples. Forty five (96%) of the 47 runs comprised 2 or 3 consecutive positives. Ninety six (52%) of 185 reported illnesses during 235 child-months were picornavirus positive (0.4/child-month); 25 infections were asymptomatic (0.11/child-month). The infection rate was highest in fall (0.66/childmonth); the winter rate (0.44/child-month) was similar to that in spring (0.5) and summer (0.43). Picornavirus infections in healthy children were common (0.51/child-month), episodic, and usually associated with brief illness; one fifth of infections were asymptomatic. The infection rate was highest in fall; infections in winter occurred at the same rate as in spring and summer. (c) 2006 Wiley-Liss, Inc.