Calcium ions promote osteogenic differentiation and mineralization of human dental pulp cells: implications for pulp capping materials
Calcium ions promote osteogenic differentiation and mineralization of human dental pulp cells: implications for pulp capping materials
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DOI：
10.1007/s10856-011-4531-0
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发表时间：
2012-03-01
影响因子：
3.7
通讯作者：
Xiao, Yin
中科院分区：
文献类型：
--
作者：
An, Shaofeng;Gao, Yan;Xiao, Yin
钙（Ca）是大多数盖髓材料的主要元素，在矿化中起着至关重要的作用。不同的盖髓材料可以释放不同浓度的钙离子，从而导致不同的临床结果。本研究的目的是探讨不同浓度的Ca离子对人牙髓细胞（hDPCs）生长和成骨分化的影响。生长培养基和成骨诱导培养基中添加不同浓度的Ca离子。使用 Cell Counting Kit-8 测定生长培养基中 hDPC 的增殖情况。通过碱性磷酸酶（ALP）测定、茜素红S/von kossa染色、Ca含量定量测定来测量成骨分化和矿化。通过定量实时聚合酶链反应（qRT-PCR）研究所选的成骨分化标记物。在1.8-16.2 mM范围内，Ca离子浓度的增加对细胞增殖没有影响，但导致成骨分化的变化。值得注意的是，钙离子浓度较高时，矿化基质结核形成增强；然而，ALP 活性和基因表达却降低了。 qRT-PCR 结果显示，Ca 离子浓度升高时，I 型胶原和 Runx2 mRNA 表达呈下调趋势，而骨桥蛋白和骨钙素 mRNA 表达显着上调。培养基中的钙离子含量可以显着影响 hDPC 的成骨特性，这表明优化牙髓盖材料中钙离子释放的重要性，以实现理想的临床结果。
Calcium (Ca) is the main element of most pulp capping materials and plays an essential role in mineralization. Different pulp capping materials can release various concentrations of Ca ions leading to different clinical outcomes. The purpose of this study was to investigate the effects of various concentrations of Ca ions on the growth and osteogenic differentiation of human dental pulp cells (hDPCs). Different concentrations of Ca ions were added to growth culture medium and osteogenic inductive culture medium. A Cell Counting Kit-8 was used to determine the proliferation of hDPCs in growth culture medium. Osteogenic differentiation and mineralization were measured by alkaline phosphatase (ALP) assay, Alizarin red S/von kossa staining, Ca content quantitative assay. The selected osteogenic differentiation markers were investigated by quantitative real-time polymerase chain reaction (qRT-PCR). Within the range of 1.8-16.2 mM, increased concentrations of Ca ions had no effect on cell proliferation, but led to changes in osteogenic differentiation. It was noted that enhanced mineralized matrix nodule formation was found in higher Ca ions concentrations; however, ALP activity and gene expression were reduced. qRT-PCR results showed a trend towards down-regulated mRNA expression of type I collagen and Runx2 at elevated concentrations of Ca ions, whereas osteopontin and osteocalcin mRNA expression were significantly up-regulated. Ca ions content in the culture media can significantly influence the osteogenic properties of hDPCs, indicating the importance of optimizing Ca ions release from dental pulp capping materials in order to achieve desirable clinical outcomes.