Order-Disorder Transitions in the Cardiac Troponin Complex.
Order-Disorder Transitions in the Cardiac Troponin Complex.
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DOI：
10.1016/j.jmb.2016.06.022
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发表时间：
2016-07-31
影响因子：
5.6
通讯作者：
Rhoades E
中科院分区：
文献类型：
--
作者：
Metskas LA;Rhoades E
肌钙蛋白复合物是一种分子开关，将细胞内钙离子浓度的变化与肌动蛋白和肌球蛋白的结合和解离联系起来，有效地允许横纹肌中的兴奋-收缩偶联。虽然肌肉生物物理学和结构生物学有着悠久的历史，但许多使肌钙蛋白功能得以实现的机制细节仍然不完全清楚。本文综述了肌钙蛋白复合物在肌肉细丝上的结构，重点是肌钙蛋白I亚基柔性区域的构象变化。特别是，我们专注于肌钙蛋白I的C-末端结构域的有序-无序转换，这在心脏疾病中具有重要意义，并且也可能具有作为耦合结合和折叠研究的模型系统的潜力。
The troponin complex is a molecular switch that ties shifting intracellular calcium concentration to association and dissociation of actin and myosin, effectively allowing excitation-contraction coupling in striated muscle. Although there is a long history of muscle biophysics and structural biology, many of the mechanistic details that enable troponin’s function remain incompletely understood. This review summarizes the current structural understanding of the troponin complex on the muscle thin filament, focusing on conformational changes in flexible regions of the troponin I subunit. In particular, we focus on order-disorder transitions in the C-terminal domain of troponin I, which have important implications in cardiac disease, and could also have potential as a model system for the study of coupled binding and folding.