Value of immunological markers in predicting responsiveness to influenza vaccination in elderly individuals
Value of immunological markers in predicting responsiveness to influenza vaccination in elderly individuals
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DOI：
10.1128/jvi.75.24.12182-12187.2001
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发表时间：
2001-12-01
影响因子：
5.4
通讯作者：
Weyand, CM
中科院分区：
文献类型：
--
作者：
Goronzy, JJ;Fulbright, JW;Weyand, CM
老年人感染流感病毒后发病和死亡的风险很高。由于老化的免疫系统能力下降，灭活病毒疫苗在老年人中效果较差。我们探讨了免疫参数是否可以预测抗流感病毒疫苗的不良反应，并可作为免疫衰老的生物学标志物。153名65岁至98岁的社区退休设施居民接种了三价流感疫苗。通过比较免疫前和免疫后28天的血凝抑制滴度来确定疫苗诱导的抗体反应。通过流式细胞术分析t细胞室的组成，并测定三个t细胞亚群CD4(+) CD45RO（+）细胞、CD4(+) CD28（空）细胞和CD8(+) CD28（空）细胞的大小。只有17%的疫苗接种者能够对所有三种疫苗成分产生抗体滴度的增加，46%的免疫个体对三种血凝素中的任何一种都没有反应。成功接种疫苗的可能性随着年龄的增长而下降，并且与特定T细胞亚群CD8(+) CD28(null) T细胞的扩增独立相关。CD4(+) CD45RO（+）记忆t细胞和CD4(+) CD28(null) t细胞亚群的大小对产生抗流感病毒抗体反应的能力没有影响。CD8(+) CD28(null) T细胞的频率是免疫功能受损的有用生物标志物，可识别抗体反应不足的个体。
Elderly individuals are at high risk for morbidity and mortality when infected with influenza virus. Vaccinations with inactivated virus are less effective in the elderly due to the declining competency of the aging immune system. We have explored whether immunological parameters predict poor anti-influenza virus vaccine responses and can be used as biological markers of immunosenescence. One hundred fifty-three residents of community-based retirement facilities aged 65 to 98 years received a trivalent influenza vaccine. Vaccine-induced antibody responses were determined by comparing hemagglutination inhibition titers before and 28 days after immunization. The composition of the T-cell compartment was analyzed by flow cytometry and the sizes of three T-cell subsets, CD4(+) CD45RO(+) cells, CD4(+) CD28(null) cells, and CD8(+) CD28(null) cells, were determined. Only 17% of the vaccine recipients were able to generate an increase in titers of antibody to all three vaccine components, and 46% of the immunized individuals failed to respond to any of the three hemagglutinins. The likelihood of successful vaccination declined with age and was independently correlated with the expansion of a particular T-cell subset, CD8(+) CD28(null) T cells. The sizes of the CD4(+) CD45RO(+) memory T-cell and CD4(+) CD28(null) T-cell subsets had no effect on the ability to mount anti-influenza virus antibody responses. Frequencies of CD8(+) CD28(null) T cells are useful biological markers of compromised immunocompetence, identifying individuals at risk for insufficient antibody responses.