Preliminary structural characterization and hypoglycemic effects of an acidic polysaccharide SERP1 from the residue of Sarcandra glabra
Preliminary structural characterization and hypoglycemic effects of an acidic polysaccharide SERP1 from the residue of Sarcandra glabra
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草珊瑚残渣酸性多糖 SERP1 的初步结构表征和降血糖作用。
DOI：
10.1016/j.carbpol.2017.08.071
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发表时间：
2017-11-15
影响因子：
11.2
通讯作者：
Gao, Xiangdong
中科院分区：
文献类型：
--
作者：
Liu, Wei;Lu, Weisheng;Gao, Xiangdong
从草珊瑚（Sarcandra glabra（Thunb.）通过水提取、脱色和离子交换层析纯化中井。通过HPLC、HPSEC-MALLS、FT-IR、NMR等方法对SERP 1的结构进行了表征。结果表明，SERP 1在水相中是一种均一的杂多糖，其绝对分子量为4.208 × 10（4）Da。SERP 1中存在1，4-连接的α-D-半乳糖醛酸、甲酯化的1，4-连接的α-D-半乳糖醛酸、1，4-连接的α-D-葡萄糖醛酸、1，5-连接的α-c-阿拉伯糖、1，3-连接的β-D-半乳糖、1，4-连接的α-D-葡萄糖、1，4，6-连接的β-D-葡萄糖、1，6-连接的β-D-葡萄糖和1，2-连接的鼠李糖。体外α-葡萄糖苷酶抑制实验表明，SERP 1的IC 50值较低，为49.01 μ g/mL，比相同浓度的阿卡波糖具有更强的α-葡萄糖苷酶抑制活性。SERP 1对2型糖尿病小鼠的治疗可减轻高血糖，增加肝脏周围组织的葡萄糖利用，抑制肝损伤。本研究为工业废渣的资源化利用提供了一种可能的探索。
An acidic polysaccharide (SERP1) was isolated from the residue of Sarcandra glabra (Thunb.) Nakai by water extraction and purified by decoloration and ion exchange chromatography. The structure of SERP1 was determined by HPLC, HPSEC-MALLS, FT-IR, and NMR. The results indicated that SERP1 was a homogeneous heteropolysaccharide with the absolute molecular weight of 4.208 x 10(4) Da in the aqueous phase. 1,4-linked alpha-D-galacturonic acid, methyl esterified 1,4-linked a-D-galacturonic acid, 1,4-linked alpha-D-glucuronic acid, 1,5-linked a-c-arabinose, 1,3-linked beta-D-galactose 1,4-linked alpha-D-glucose, 1,4,6-linked beta-D-glucose, 1,6-linked beta-D-glucose, and 1,2-linked rhamnose existed in SERP1. In vitro alpha-glucosidase inhibition assay indicated that SERP1 had a low IC50 value of 49.01 mu g/mL, which exhibited stronger alpha-glucosidase enzyme inhibitory activity than acarbose at the same concentration. The treatment of SERP1 to type 2 diabetes mellitus mice alleviated the hyperglycemia, increased glucose utilization of peripheral tissues of the liver and inhibited the liver injury. This study provides a possible exploration to use valuable industrial waste.