Modified Sawhorse Waveform for the Voltammetric Detection of Oxytocin
Modified Sawhorse Waveform for the Voltammetric Detection of Oxytocin
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DOI：
10.1149/1945-7111/ac4aae
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发表时间：
2022-01-01
影响因子：
3.9
通讯作者：
Zestos, Alexander G.
中科院分区：
文献类型：
--
作者：
Liu, Favian A.;Ardabili, Negar;Zestos, Alexander G.
在过去的几十年里，碳纤维微电极(CFME)被用来利用快速扫描循环伏安法(FSCV)检测神经递质和其他生物分子。这项技术测量神经递质，如多巴胺，以及最近的生理相关神经肽。催产素是一种多效性多肽激素，对适应、发育、生殖和社会行为具有重要的生理意义。这种神经肽作为压力应对分子、抗炎剂和抗氧化剂发挥作用，特别是在逆境或创伤时具有保护作用。在这里，我们使用改进的锯切波(MSW)测量酪氨酸，从而提高了对氨基酸和催产素多肽的电极灵敏度。应用MSW，减少了表面污垢，并实现了与其他单胺的联合检测。由于催产素中含有酪氨酸，因此也可以用城市生活垃圾来检测催产素。催产素检测的灵敏度为3.99+/-0.49nAmuM-1(n=5)。此外，我们还证明，在CFME上应用MSW可以实时测量大鼠脑片上外源性应用的催产素。这些研究可能成为快速、亚秒级催产素检测的新方法，对活体测量和进一步了解催产素的生理作用具有潜在的意义。
Carbon fiber microelectrodes (CFMEs) have been used to detect neurotransmitters and other biomolecules using fast-scan cyclic voltammetry (FSCV) for the past few decades. This technique measures neurotransmitters such as dopamine and, more recently, physiologically relevant neuropeptides. Oxytocin, a pleiotropic peptide hormone, is physiologically important for adaptation, development, reproduction, and social behavior. This neuropeptide functions as a stress-coping molecule, an anti-inflammatory agent, and serves as an antioxidant with protective effects especially during adversity or trauma. Here, we measure tyrosine using the Modified Sawhorse Waveform (MSW), enabling enhanced electrode sensitivity for the amino acid and oxytocin peptide. Applying the MSW, decreased surface fouling and enabled codetection with other monoamines. As oxytocin contains tyrosine, the MSW was also used to detect oxytocin. The sensitivity of oxytocin detection was found to be 3.99 +/- 0.49 nA mu M-1, (n = 5). Additionally, we demonstrate that applying the MSW on CFMEs allows for real time measurements of exogenously applied oxytocin on rat brain slices. These studies may serve as novel assays for oxytocin detection in a fast, sub-second timescale with possible implications for in vivo measurements and further understanding of the physiological role of oxytocin.