Genotoxic effects of two nickel-compounds in somatic cells of Drosophila melanogaster
Genotoxic effects of two nickel-compounds in somatic cells of Drosophila melanogaster
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DOI：
10.1016/j.mrgentox.2010.10.008
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发表时间：
2011-01-10
影响因子：
1.9
通讯作者：
Marcos, R.
中科院分区：
文献类型：
--
作者：
Carmona, Erico R.;Creus, Amadeu;Marcos, R.
鉴于镍的体内致突变和共致突变活性的可用信息很少，通过测量两个不同的遗传终点，在果蝇中评估了两种镍化合物氯化镍（NiCl 2）和硫酸镍（NiSO 4）的遗传毒性潜力。一方面，我们使用了翅斑试验，该试验基于两个合适的隐性标记多翅毛（mwh）和flar-3（flr（3））的杂合性缺失可以导致幼虫细胞的成虫盘中突变克隆的形成的原理。另一方面，体内彗星试验，检测单，双链DNA断裂，也用于幼虫血细胞。这些细胞提供了几个优点：它们对遗传毒性剂高度敏感，采样和处理方法相当简单，基础DNA损伤水平相对较低。没有显着增加的频率的三个类别的突变点（即小的单点，大的单点，和双点）中观察到的翅斑试验：然而，硫酸镍诱导显着的剂量依赖性增加的DNA损伤的彗星试验。此外，γ-辐射和NiCl 2和NiSO 4的组合处理显示出轻微的，但显着增加的频率相比，由γ-辐射单独诱导的突变点的三个类别的频率，这表明两种镍化合物具有协同作用。这些结果支持了这两种镍化合物都可以作为干扰DNA修复过程的共诱变剂的假设，并且体内彗星试验是检测NiSO_4诱导的D.黑腹菌(C)2010爱思唯尔有限公司版权所有。
In view of the scarcely available information on the in vivo mutagenic and co-mutagenic activity of nickel, the genotoxic potential of two nickel-compounds, nickel chloride (NiCl2) and nickel sulphate (NiSO4), was assessed in Drosophila melanogaster by measuring two different genetic endpoints. On the one hand, we used the wing-spot assay, which is based on the principle that the loss of heterozygosity of two suitable recessive markers, multiple wing hairs (mwh) and flare-3 (flr(3)), can lead to the formation of mutant clones in the imaginal disks of larval cells. On the other hand, the in vivo comet assay, which detects single- and double-strand DNA breaks, was also used with larval haemocytes. These cells offer several advantages: they are highly sensitive to genotoxic agents, the sampling and processing methodologies are quite simple and the level of basal DNA damage is relatively low. No significant increases in the frequencies of the three categories of mutant spots (i.e. small single spots, large single spots, and twin spots) were observed in the wing-spot assay: however, NiSO4 induced significant dose-dependent increases in DNA damage in the comet assay. In addition, the combined treatments with gamma-radiation and NiCl2 and NiSO4 showed a slight but significant increase in the frequency of the three categories of mutant spots compared with the frequency induced by gamma-radiation alone, indicating that both nickel compounds have a synergistic interaction. These results support the assumption that both nickel compounds could act as co-mutagens interfering with DNA-repair processes and that the in vivo comet assay is a sensitive and effective method for detecting the DNA damage induced by NiSO4 in haemocytes of D. melanogaster. (C) 2010 Elsevier B.V. All rights reserved.