Presence of poly(A) and poly(A)-rich tails in a positive-strand RNA virus known to lack 3 ' poly(A) tails
Presence of poly(A) and poly(A)-rich tails in a positive-strand RNA virus known to lack 3 ' poly(A) tails
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已知缺乏 3 ' Poly(A) 尾的正链 RNA 病毒中存在 Poly(A) 和富含 Poly(A) 尾
DOI：
10.1016/j.viro1.2014.02.002
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Shirong Jia
中科院分区：
文献类型：
--
作者：
Weimin Li;Yongqiang Zhang;Chao Zhang;Xinwu Pei;Zhixing Wang;Shirong Jia
在这里，我们发现烟草花叶病毒（TMV），一种正链RNA病毒，已知以3个tRNA样结构结束，确实拥有一小部分带有聚腺苷酸尾巴的gRNA。特别地，许多尾位于对应于近全长gRNA的3 ′端的位点处，并且由富含聚（A）的序列组成，所述富含聚（A）的序列含有除腺苷之外的其他核苷酸，类似于在所有生物界中观察到的降解刺激聚（A）尾。进一步的研究表明，这些聚腺苷酸化的RNA物种在叶绿体中不富集。cpPNIPs是一种叶绿体定位的多核苷酸聚合酶，已知它不仅能使富含poly（A）的尾区变窄，而且还能作为3 - 5 bp的核糖核酸外切酶，它的沉默不会改变与TMV RNA相关的聚腺苷酸尾区的分布。然而，由于在其他缺乏poly（A）尾的遗传学上不同的正链RNA病毒中也检测到类似的尾，这种聚腺苷酸化可能反映了宿主和病毒之间常见但未知的界面。
Here we show thatTobacco mosaic virus(TMV), a positive-strand RNA virus known to end with 3׳ tRNA-like structures, does possess a small fraction of gRNA bearing polyadenylate tails. Particularly, many tails are at sites corresponding to the 3׳ end of near full length gRNA, and are composed of poly(A)-rich sequences containing the other nucleotides in addition to adenosine, resembling the degradation-stimulating poly(A) tails observed in all biological kingdoms. Further investigations demonstrate that these polyadenylated RNA species are not enriched in chloroplasts. Silencing of cpPNPase, a chloroplast-localized polynucleotide polymerase known to not only polymerize the poly(A)-rich tails but act as a 3׳ to 5׳ exoribonuclease, does not change the profile of polyadenylate tails associated with TMV RNA. Nevertheless, because similar tails were also detected in other phylogenetically distinct positive-strand RNA viruses lacking poly(A) tails, such kind of polyadenylation may reflect a common but as-yet-unknown interface between hosts and viruses.