Periostin deletion suppresses late-phase response in mouse experimental allergic conjunctivitis
Periostin deletion suppresses late-phase response in mouse experimental allergic conjunctivitis
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DOI：
10.1016/j.alit.2018.09.007
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发表时间：
2019-04-01
影响因子：
6.8
通讯作者：
Matsuda, Akira
中科院分区：
文献类型：
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作者：
Asada, Yosuke;Okano, Mikiko;Matsuda, Akira
背景资料：为了探讨骨膜蛋白（POSTN），细胞外基质优先表达的Th 2-歪斜的条件下，在过敏性结膜炎的病理生理学的潜在作用：POSTN的作用豚草诱导的实验性过敏性结膜炎（RW-EAC）进行了评估使用POSTN基因敲除（KO）和同源BALB/c野生型小鼠。进行组织学分析以计数结膜组织中的嗜酸性粒细胞/嗜碱性粒细胞。采用定量聚合酶链反应（Q-PCR）检测RW-EAC模型中POSTN-KO和野生型小鼠Th 2细胞因子的表达，并采用基因芯片技术分析RW-EAC模型中POSTN-KO和野生型小鼠Th 2细胞因子表达差异。与野生型小鼠相比，POSTN-KO小鼠中RW-EAC结膜中浸润性嗜酸性粒细胞的数量减少。结膜组织的Q-PCR分析显示，使用POSTN-KO小鼠，在RW-EAC中诱导Th 2细胞因子（Ccl 5、Il 4、Il 5、Il 13）表达，并且在RW-EAC的结膜中减弱Ccl 5、Il 4、Il 13 mRNA表达。微阵列分析和免疫组化分析表明，减少嗜碱性粒细胞标志物（Mcpt 8）的表达和减少的浸润嗜碱性粒细胞在结膜的RW-EAC在POSTN-KO mice.Conclusions：POPT 8在结膜组织中的表达起着不可或缺的作用，在后期反应的RW-EAC模型，通过促进嗜酸性粒细胞/嗜碱性粒细胞浸润和增强Th 2细胞因子的表达。Copyright（C）2019，日本变态反应学会制作和主办：Elsevier B. V.
Background: To investigate the potential roles of periostin (POSTN), an extracellular matrix preferentially expressed in Th2-skewed conditions in the pathophysiology of allergic conjunctivitis.Methods: The roles of POSTN in ragweed-induced experimental allergic conjunctivitis (RW-EAC) were evaluated using both POSTN-knockout (KO) and congenic BALB/c wild-type mice. Histological analysis was carried out to enumerate eosinophils/basophils in the conjunctival tissue. Th2 cytokine expression was evaluated by quantitative polymerase chain reaction (Q-PCR), and microarray analysis was performed to elucidate genes differentially expressed in POSTN-KO and wild-type mice in the RW-EAC model.Results: Upregulation of POSTN expression and eosinophil infiltration was observed in subconjunctival tissue of RW-EAC in the wild-type mice. The number of infiltrating eosinophils in the conjunctivae of RW-EAC was diminished in POSTN-KO mice compared to wild-type mice. Q-PCR analysis of conjunctival tissue showed induction of Th2 cytokine (Ccl5, Il4, Il5, Il13) expression in the RW-EAC and attenuated Cc15, Il4, Il13 mRNA expression in the conjunctivae of the RW-EAC using POSTN-KO mice. Microarray analysis and immunohistochemical analysis showed diminished basophil marker (Mcpt8) expression and reduced numbers of infiltrating basophils in the conjunctivae of RW-EAC in POSTN-KO mice.Conclusions: POSTN expression in conjunctival tissue plays an indispensable role in the late-phase reaction of the RW-EAC model by facilitating eosinophil/basophil infiltration and augmenting Th2 cytokine expression. Copyright (C) 2019, Japanese Society of Allergology. Production and hosting by Elsevier B.V.