Effect of Transplantation of Mesenchymal Stem Cells Induced into Early Hepatic Cells in Streptozotocin-Induced Diabetic Mice
Effect of Transplantation of Mesenchymal Stem Cells Induced into Early Hepatic Cells in Streptozotocin-Induced Diabetic Mice
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DOI：
10.1248/bpb.33.1212
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发表时间：
2010-07-01
影响因子：
2
通讯作者：
Soleimani, Masoud
中科院分区：
文献类型：
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作者：
Dinarvand, Peyman;Hashemi, Seyed Mahmoud;Soleimani, Masoud
糖尿病的细胞替代疗法受到广泛关注。本研究探讨了经诱导的骨髓间充质干细胞（BM-MSCs）移植到链脲佐菌素（STZ）诱导的糖尿病小鼠的内胚层和早期肝细胞中的作用。将小鼠BM-MSC在肝细胞生长因子（HGF）和成纤维细胞生长因子（FGF-4）存在下培养2周并移植到糖尿病小鼠中。观察各组大鼠血糖、腹腔葡萄糖耐量试验、血清胰岛素、体重及胰岛组织学变化。结果表明，移植同系诱导的MSCs可以逆转STZ诱导的小鼠糖尿病。对高血糖和胰岛破坏的小鼠进行治疗导致胰岛修复。诱导骨髓间充质干细胞（iBM-MSCs）组的血糖、腹腔葡萄糖耐量试验和血清胰岛素水平明显恢复。此外，与其他组相比，iBM-MSCs组的体重和胰岛数量显著增加。结果表明，BM-MSCs诱导为内胚层和早期肝细胞是合适的候选人，以细胞为基础的治疗糖尿病。
Cellular replacement therapy for diabetes mellitus has received much attention. In this study we investigated the effect of transplantation of bone marrow-derived mesenchymal stem cells (BM-MSCs) induced into endoderm and early hepatic cells in streptozotocin (STZ)-induced diabetic mice. Mouse BM-MSCs were cultured in the presence of hepatocyte growth factor (HGF) and fibroblast growth factor (FGF-4) for 2 weeks and transplanted into diabetic mice. Blood glucose levels, intraperitoneal glucose tolerance test, serum insulin, body weight and islets histology were analyzed. The results demonstrated that transplantation of syngeneic induced MSCs could reverse STZ-induced diabetes in mice. The treatment of mice with hyperglycemia and islet destruction resulted in the repair of pancreatic islets. Blood glucose levels, intraperitoneal glucose tolerance test, and serum insulin were significantly recovered in induced BM-MSCs (iBM-MSCs) group. In addition, in the iBM-MSCs group the body weight and the number of islets were significantly increased compared to other groups. The results demonstrate that BM-MSCs induced into endoderm and early hepatic cells are suitable candidates for cell-based therapy of diabetes mellitus.