Spatiotemporal rescue of memory dysfunction in Drosophila
Spatiotemporal rescue of memory dysfunction in Drosophila
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DOI：
10.1126/science.1089035
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发表时间：
2003-12-05
期刊：
影响因子：
56.9
通讯作者：
Davis, RL
中科院分区：
文献类型：
--
作者：
McGuire, SE;Le, PT;Davis, RL
我们在果蝇中开发了一种时间和区域基因表达靶向（TARGET）方法，并展示了同时在空间和时间上拯救记忆缺陷。芜菁编码腺苷酸环化酶在成年大脑蘑菇体中的短暂表达是必要的，足以挽救芜菁记忆缺陷，这排除了这种缺陷的病因是发育性脑缺陷，并证明了芜菁在这些神经元记忆形成中的急性作用。TARGET系统在同时解决何时何地需要基因产物的问题方面提供了通用的实用程序。
We have developed a method for temporal and regional gene expression targeting (TARGET) in Drosophila and show the simultaneous spatial and temporal rescue of a memory defect. The transient expression of the rutabaga-encoded adenylyl cyclase in the mushroom bodies of the adult brain was necessary and sufficient to rescue the rutabaga memory deficit, which rules out a developmental brain defect in the etiology of this deficit and demonstrates an acute role for rutabaga in memory formation in these neurons. The TARGET system offers general utility in simultaneously addressing issues of when and where gene products are required.