Interferon induction by viruses. VI. Reovirus: virion genome dsRNA as the interferon inducer in aged chick embryo cells.
Interferon induction by viruses. VI. Reovirus: virion genome dsRNA as the interferon inducer in aged chick embryo cells.
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病毒诱导干扰素。
DOI：
10.1089/jir.1980.1.155
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发表时间：
1980
期刊：
影响因子：
--
通讯作者：
Marcus,PI
中科院分区：
文献类型：
--
作者：
Winship,TR;Marcus,PI
通过分析剂量（多重性）-反应（干扰素产量）曲线，测定了禽呼肠孤病毒和人呼肠孤病毒在老化鸡胚细胞中的干扰素诱导颗粒（IFP）活性。这些曲线最佳地拟合了一个模型，其中每个被≥ 1个IFP感染的细胞产生量子产量的干扰素。禽呼肠孤病毒原液含有多达60倍以上的IFP比空斑形成颗粒（PFP）。在UV照射后，IFP：PFP的比率变为197，这表明实际上禽呼肠孤病毒的每个物理颗粒都可以作为干扰素诱导颗粒起作用。因此，大约三分之一的非感染性颗粒本质上是IFP，而另外三分之二可以在最佳剂量的UV辐射下转化为IFP状态，相当于9.4次致命撞击，即，8000尔格/平方毫米。紫外线照射的禽呼肠孤病毒诱导的干扰素产量约为每个细胞通常产量的两倍。野生型人呼肠孤病毒（3型）和突变体201（A，RNA+）和ts 447（C，RNA-）是干扰素的优秀诱导剂，但只有大约三分之一的感染颗粒具有干扰素诱导颗粒的功能，这意味着几乎所有物理颗粒都无法发挥IFP的作用。紫外线照射人呼肠孤病毒导致IFP活性略有下降，我们的数据支持这样的假设，即病毒粒子基因组dsRNA构成了禽呼肠孤病毒的干扰素诱导剂部分，并且在其容许宿主细胞中，基因组dsRNA从大多数粒子到细胞质中的推定识别位点的加工以高概率自然发生。对于人呼肠孤病毒，这是一个罕见得多的事件，可能是固有的，只有感染性病毒，并可能需要有限的转录表达。禽呼肠孤病毒老化的鸡胚细胞系统的敏感性，建议进一步研究干扰素诱导的机制，病毒粒子含有预先存在的dsRNA。
The interferon-inducing particle (IFP) activity of avian and human reoviruses in aged chick embryo cells was determined by analyzing dose (multiplicity)-response (interferon yield) curves. These curves fit best a model in which each cell infected with ≥ 1 IFP producces a quantum yield of interferon. Avian reovirus stocks contained as many as 60 times more IFP than plaque-forming particles (PFP). Upon UV-irradiation the ratio of IFP:PFP became 197, suggesting that virtually every physical particle of avian reovirus could function as an interferon-inducing particle. Thus, about one-third of the noninfectious particles were intrinsically IFP and the other two-thirds could be converted to IFP status at an optimal dose of UV radiation, the equivalent of 9.4 lethal hits, i.e., 8000 ergs/mm2. UV-irradiated avian reovirus induced about twice the usual yield of interferon on a per cell basis.Wildtype human reovirus (type 3) and mutantsts201(A,RNA+) andts447(C,RNA−) were excellent inducers of interferon, but only about 1 in 3 infectious particles functioned as an interferon-inducing particle, meaning that virtually all physical particles failed to function as IFP. UV-irradiation of human reoviruses resulted in a slight loss of IFP activity.Our data support the hypothesis that virion genome dsRNA constitutes the interferon inducer moiety of avian reoviruses and that in its permissive host cell the processing of genome dsRNA from most particles to a putative recognition site in the cytoplasm occurs naturally with a high probability. For human reovirus this is a much rarer event which may be intrinsic only to infectious virus, and may require limited transcription for expression.The sensitivity of the avian reovirus-aged chick embryo cell system recommends it for further study on the mechanism of interferon induction by virions containing pre-existing dsRNA.