STC1 regulates glioblastoma migration and invasion via the TGF-β/SMAD4 signaling pathway
STC1 regulates glioblastoma migration and invasion via the TGF-β/SMAD4 signaling pathway
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DOI：
10.3892/mmr.2019.10579
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发表时间：
2019-10-01
影响因子：
3.4
通讯作者：
Wang, Qibai
中科院分区：
文献类型：
--
作者：
Xiong, Yan;Wang, Qibai
Stanniocalcin-1（STC 1）参与癌症进展;然而，STC 1在胶质母细胞瘤中的功能仍然未知。本研究采用免疫组织化学方法检测STC 1蛋白在胶质母细胞瘤中的表达水平。通过蛋白质印迹法评估STC 1沉默后STC 1、SMAD 2/3和SMAD 4蛋白的表达水平。进行EdU和Transwell测定以确定细胞的增殖和迁移能力。采用定量PCR方法检测STC 1、SMAD 4和microRNA（miR）-34a的mRNA表达水平。STC 1在胶质母细胞瘤组织中的表达水平升高。STC 1与胶质母细胞瘤患者的不良预后显著相关（P
Stanniocalcin-1 (STC1) is involved in cancer progression; however, the function of STC1 in glioblastoma remains unknown. In the present study, the expression levels of STC1 protein in glioblastoma were detected using immunohistochemistry. The expression levels of STC1, SMAD2/3 and SMAD4 proteins, following silencing of STC1, were assessed via western blotting. EdU and Transwell assays were performed to determine the proliferation and migration ability of the cells. The mRNA expression levels of STC1, SMAD4 and microRNA (miR)-34a were determined using quantitative PCR. The expression levels of STC1 were increased in glioblastoma tissues. STC1 revealed a significant association with poor outcome in patients with glioblastoma (P