Elevated levels of mRNA can account for the trans-activation of human immunodeficiency virus.
Elevated levels of mRNA can account for the trans-activation of human immunodeficiency virus.
复制标题
mRNA 水平升高可以解释人类免疫缺陷病毒的反式激活。
DOI：
10.1073/pnas.83.24.9734
复制
发表时间：
1986
影响因子：
11.1
通讯作者：
Walker,MD
中科院分区：
文献类型：
--
作者：
Peterlin,BM;Luciw,PA;Barr,PJ;Walker,MD
The genome of human immunodeficiency virus encodes a protein that dramatically elevates amounts of viral proteins. The precise mechanism of this trans-activation remains to be established. It has been reported that trans-activation can occur without major changes in the levels of mRNA. We constructed recombinant plasmids containing those viral sequences required in cis for trans-activation linked to the chloramphenicol acetyltransferase gene. These plasmids were introduced into cultured cells in either the presence or absence of a second plasmid that directed expression of the viral trans-activator protein. Expression of the chloramphenicol acetyltransferase gene was measured at the level of protein (by enzymatic assay) and RNA (by ribonuclease protection and primer extension). Our results demonstrate that trans-activation is accompanied by large increases in mRNA levels; these increases may be sufficient to explain the elevated levels of trans-activated protein.
