An Evaluation of Acute Toxicity of Colloidal Silver Nanoparticles
An Evaluation of Acute Toxicity of Colloidal Silver Nanoparticles
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DOI：
10.1292/jvms.11-0038
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发表时间：
2011-11-01
影响因子：
1.2
通讯作者：
Kaewamatawong, Theerayuth
中科院分区：
文献类型：
--
作者：
Maneewattanapinyo, Pattwat;Banlunara, Wijit;Kaewamatawong, Theerayuth
根据OECD指南，在实验室动物中进行了胶体银纳米颗粒（AgNP）的急性经口毒性、眼刺激、腐蚀和皮肤毒性试验。在整个观察期内，以5，000 mg/kg的有限剂量口服给予AgNP既不产生死亡也不产生急性毒性体征。小鼠的体重增加百分比在对照组和处理组之间没有显示出显著差异。在血液学分析中，用AgNP处理的小鼠和对照之间没有显著差异。血液化学分析也显示检查的任何参数均无差异。在各器官中未观察到任何大体病变或组织病理学变化。结果表明，胶体银纳米颗粒的LD 50大于5，000 mg/kg体重。在急性眼刺激和腐蚀研究中，在72小时的观察期内，当将不同剂量的胶体AgNPs滴入豚鼠眼中时，未观察到死亡和毒性体征。然而，滴注5，000 ppm的AgNP在早期24小时观察时间内产生短暂的眼刺激。在暴露于不同剂量的胶体AgNPs的豚鼠皮肤中未观察到任何大体异常。此外，显微镜下未观察到与皮肤组织变化相关的显著AgNP暴露。总之，本研究中所有毒性试验的这些结果表明，胶体AgNP在短期内施用于动物模型的口腔、眼睛和皮肤时可能相对安全。
Tests for acute oral toxicity, eye irritation, corrosion and dermal toxicity of colloidal silver nanoparticles (AgNPs) were conducted in laboratory animals following OECD guidelines. Oral administration of AgNPs at a limited dose of 5,000 mg/kg produced neither mortality nor acute toxic signs throughout the observation period. Percentage of body weight gain of the mice showed no significant difference between control and treatment groups. In the hematological analysis, there was no significant difference between mice treated with AgNPs and controls. Blood chemistry analysis also showed no differences in any of the parameter examined. There was neither any gross lesion nor histopathological change observed in various organs. The results indicated that the LD50 of colloidal AgNPs is greater than 5,000 mg/kg body weight. In acute eye irritation and corrosion study, no mortality and toxic signs were observed when various doses of colloidal AgNPs were instilled in guinea pig eyes during 72 hr observation period. However, the instillation of AgNPs at 5,000 ppm produced transient eye irritation during early 24 hr observation time. No any gross abnormality was noted in the skins of the guinea pigs exposed to various doses of colloidal AgNPs. In addition, no significant AgNPs exposure relating to dermal tissue changes was observed microscopically. In summary, these findings of all toxicity tests in this study suggest that colloidal AgNPs could be relatively safe when administered to oral, eye and skin of the animal models for short periods of time.