Intravital imaging of a spheroid-based orthotopic model of melanoma in the mouse ear skin.
Intravital imaging of a spheroid-based orthotopic model of melanoma in the mouse ear skin.
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DOI：
10.4161/intv.25805
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发表时间：
2013-01-01
期刊：
影响因子：
--
通讯作者：
Bear, James E
中科院分区：
文献类型：
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作者：
Chan, Keefe T;Jones, Stephen W;Bear, James E
多光子显微镜是一种强大的工具，它能够在体内显示荧光标记的肿瘤细胞及其间质相互作用。我们开发了一种原位模型，将多细胞黑色素瘤肿瘤球体植入小鼠耳朵皮肤的真皮，而不需要进行侵入性手术。在这里，我们展示了这种方法在观察原发肿瘤、单细胞肌动蛋白动力学和肿瘤相关血管系统方面的应用。这些方法可以广泛应用于研究一系列关于体内肿瘤细胞行为的生物学问题。
Multiphoton microscopy is a powerful tool that enables the visualization of fluorescently tagged tumor cells and their stromal interactions within tissues in vivo. We have developed an orthotopic model of implanting multicellular melanoma tumor spheroids into the dermis of the mouse ear skin without the requirement for invasive surgery. Here, we demonstrate the utility of this approach to observe the primary tumor, single cell actin dynamics, and tumor-associated vasculature. These methods can be broadly applied to investigate an array of biological questions regarding tumor cell behavior in vivo.