Characterization of B and plasma cells in blood, bone marrow, and secondary lymphoid organs of rhesus macaques by multicolor flow cytometry
Characterization of B and plasma cells in blood, bone marrow, and secondary lymphoid organs of rhesus macaques by multicolor flow cytometry
复制标题
DOI：
10.1189/jlb.1hi0514-243r
复制
发表时间：
2015-01-01
影响因子：
5.5
通讯作者：
Stahl-Hennig, Christiane
中科院分区：
文献类型：
--
作者：
Neumann, Berit;Klippert, Antonina;Stahl-Hennig, Christiane
B细胞作为体液免疫应答的重要部分，在BM中产生，迁移到次级淋巴器官，并且在活化时，分化成产生抗体的记忆B细胞或浆细胞。尽管它们在不同疾病中发挥着关键作用，但作为各种人类疾病有价值模型的健康恒河猴的全面表征仍然缺失。使用多参数流式细胞术，我们分析了从两个位置收集的BM中的B细胞，即，髂嵴（BMca）和股骨（BMfem）、PB以及健康恒河猴的次级淋巴器官。我们评估了未成熟和成熟B细胞以及CD 19（+）CD 20-CD 38（+）/（++）CD 138（+）/（++）浆母细胞/浆细胞的频率。此外，我们还发现了B细胞活化和增殖、趋化因子受体和免疫球蛋白以及记忆B细胞亚群分布的标志物表达的位点特异性差异。由于次级淋巴器官中幼稚B细胞的频率最高，因此与外周和BM中的B细胞相比，CD 80、CD 95和Ki 67的表达较低，而IgD、CXCR 4（CD 184）和CCR 7（CD 197）的表达较高。有趣的是，BMca与BMfem在B细胞的频率、它们的CD 80和CXCR 4、T细胞和浆细胞的表达方面不同。总之，这些数据确定了上述参数的基线值，并为未来研究不同疾病中的B和浆细胞提供了基础。
B cells, as an important part of the humoral immune response, are generated in the BM, migrate to secondary lymphoid organs, and upon activation, differentiate into antibody-producing memory B cells or plasma cells. Despite the pivotal roles that they play in different diseases, a comprehensive characterization in healthy rhesus macaques, which serve as valuable models for a variety of human diseases, is still missing. With the use of multiparameter flow cytometry, we analyzed B cells in BM collected from two locations, i.e., the iliac crest (BMca) and the femur (BMfem), PB, as well as secondary lymphoid organs of healthy rhesus macaques. We assessed the frequencies of immature and mature B cells, as well as CD19(+) CD20-CD38(+)/(++) CD138(+)/(++) plasmablasts/plasma cells. Furthermore, we found site-specific differences in the expression of markers for B cell activation and proliferation, chemokine receptors and Igs, as well as the distribution of memory B cell subpopulations. As secondary lymphoid organs harbor the highest frequencies of naive B cells, expression of CD80, CD95, and Ki67 was lower compared with B cells in the periphery and BM, whereas expression of IgD, CXCR4 (CD184), and CCR7 (CD197) was higher. Interestingly, BMca differed from BMfem regarding frequencies of B cells, their expression of CD80 and CXCR4, T cells, and plasma cells. In summary, these data identify baseline values for the above-mentioned parameters and provide the foundation for future studies on B and plasma cells in different diseases.