Production of poly(3-hydroxybutyrate) by fed-batch culture of recombinant Escherichia coli with a highly concentrated whey solution
Production of poly(3-hydroxybutyrate) by fed-batch culture of recombinant Escherichia coli with a highly concentrated whey solution
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DOI：
10.1128/aem.66.8.3624-3627.2000
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发表时间：
2000-08-01
影响因子：
4.4
通讯作者：
Lee, SY
中科院分区：
文献类型：
--
作者：
Ahn, WS;Park, SJ;Lee, SY
研究了携带产碱杆菌聚羟基烷酸(PHA)生物合成基因的重组大肠杆菌CGSC 4401以乳清为原料生产聚3-羟基丁酸酯(PHB)的发酵工艺。用每升含280g乳糖当量的浓缩乳清液对含有pJC4的大肠杆菌CGSC 4401进行分批补料培养。最终菌体浓度为119.5 g/L，PHB浓度为96.2g/L，发酵时间为37.5h，PHB产量为2.57g/L/h。
Fermentation strategies for the production of poly(3-hydroxybutyrate) (PHB) from whey by recombinant Escherichia coli strain CGSC 4401 harboring the Alcaligenes latus polyhydroxyalkanoate (PHA) biosynthesis genes were developed. The pa-stat fed-batch cultures of E. coli CGSC 4401 harboring pJC4, a stable plasmid containing the ii, latus PHA biosynthesis genes, were carried out with a concentrated whey solution containing 280 g of lactose equivalent per liter. Final cell and PHB concentrations of 119.5 and 96.2 g/liter, respectively, were obtained in 37.5 h, which resulted in PHB productivity; of 2.57 g/liter/h.