Electrostatic interaction effects on tension-induced pore formation in lipid membranes
Electrostatic interaction effects on tension-induced pore formation in lipid membranes
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DOI：
10.1103/physreve.92.012708
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发表时间：
2015-07-06
影响因子：
2.4
通讯作者：
Yamazaki, Masahito
中科院分区：
文献类型：
--
作者：
Karal, Mohammad Abu Sayem;Levadnyy, Victor;Yamazaki, Masahito
我们研究了静电相互作用对张力诱导巨单层囊泡脂质膜在恒定外加张力下形成孔的速率常数（k（p））的影响。溶液中的盐浓度的降低以及膜的表面电荷密度的增加增加kp。这些数据表明，k（p）随着静电相互作用程度的增加而增加。我们开发了一个理论上的静电相互作用对自由能分布的膜含有一个prepore和K（P）的值的影响，这个理论解释了实验结果，并符合实验数据合理地存在弱静电相互作用。基于这些结果，我们得出结论，由于静电相互作用的预孔状态的自由能垒的降低是导致k（p）增加的主要因素。
We investigated the effects of electrostatic interactions on the rate constant (k(p)) for tension-induced pore formation in lipid membranes of giant unilamellar vesicles under constant applied tension. A decrease in salt concentration in solution as well as an increase in surface charge density of the membranes increased kp. These data indicate that k(p) increases as the extent of electrostatic interaction increases. We developed a theory on the effect of the electrostatic interactions on the free energy profile of the membrane containing a prepore and also on the values of k(p); this theory explains the experimental results and fits the experimental data reasonably well in the presence of weak electrostatic interactions. Based on these results, we conclude that a decrease in the free energy barrier of the prepore state due to electrostatic interactions is the main factor causing an increase in k(p).