Electrospun Microfiber Membranes Embedded with Drug-Loaded Clay Nanotubes for Sustained Antimicrobial Protection
Electrospun Microfiber Membranes Embedded with Drug-Loaded Clay Nanotubes for Sustained Antimicrobial Protection
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嵌入载药粘土纳米管的电纺微纤维膜可提供持续的抗菌保护
DOI：
10.1021/nn506255e
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发表时间：
2015-02-01
期刊：
影响因子：
17.1
通讯作者：
Lvov, Yuri
中科院分区：
文献类型：
--
作者：
Xue, Jiajia;Niu, Yuzhao;Lvov, Yuri
通过静电纺丝将载药的高岭土纳米管掺杂到聚（己内酯）/明胶微纤维中，制备了具有持续给药功能的引导组织再生/引导骨再生膜。在纤维膜中使用20 wt %的纳米管含量，允许在膜中装载25 wt %的甲硝唑药物。将直径为50 nm、长度为600 nm的纳米管排列在直径为400 nm的静电纺丝纤维中，使膜沿收集器旋转方向的拉伸强度增加了一倍。掺高岭石的膜对细胞向内生长起屏障作用，具有良好的生物相容性。将甲硝唑负载的高岭土纳米管掺入到微纤维中，可以延长药物释放时间超过20天，而直接掺入微纤维中则为4天。甲硝唑从膜上的持续释放阻止了厌氧梭杆菌的定植，而真核细胞仍然可以附着在载药复合膜上并增殖。这表明高岭土粘土纳米管作为药物容器的潜力，可以结合到电纺丝膜中用于临床应用。
Guided tissue regeneration/guided bone regeneration membranes with sustained drug delivery were developed by electrospinning drug-loaded halloysite clay nanotubes doped into poly(caprolactone)/gelatin microfibers. Use of 20 wt % nanotube content in fiber membranes allowed for 25 wt % metronidazole drug loading in the membrane. Nanotubes with a diameter of 50 nm and a length of 600 nm were aligned within the 400 nm diameter electrospun fibers, resulting in membranes with doubling of tensile strength along the collector rotating direction. The halloysite-doped membranes acted as barriers against cell ingrows and have good biocompatibility. The metronidazole-loaded halloysite nanotubes incorporated in the microfibers allowed for extended release of the drugs over 20 days, compared to 4 days when directly admixed into the microfibers. The sustained release of metronidazole from the membranes prevented the colonization of anaerobic Fusobacteria, while eukaryotic cells could still adhere to and proliferate on the drug-loaded composite membranes. This indicates the potential of halloysite clay nanotubes as drug containers that can be incorporated into electrospun membranes for clinical applications.