Staphylococcus aureus ST6-t701 Isolates from Food-Poisoning Outbreaks (2006-2013) in Xi'an, China
Staphylococcus aureus ST6-t701 Isolates from Food-Poisoning Outbreaks (2006-2013) in Xi'an, China
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从中国西安食物中毒疫情（2006-2013 年）中分离出金黄色葡萄球菌 ST6-t701
DOI：
10.1089/fpd.2014.1850
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发表时间：
2015-03-01
影响因子：
2.8
通讯作者：
Wang, Xin
中科院分区：
文献类型：
--
作者：
Li, Guanghui;Wu, Shouzhi;Wang, Xin
本研究的目的是调查2006 - 2013年西安市7起葡萄球菌食物中毒（SFP）中分离的金黄色葡萄球菌的分子流行病学。一共七个S。对7起SFP暴发的金黄色葡萄球菌进行多位点序列分型、SPA分型、脉冲场凝胶电泳（PFGE）、药敏、毒素和耐药基因鉴定。结果表明，S.金黄色葡萄球菌ST 6-t701（71.4%）是最主要的克隆，其次是ST 5-t002和ST 59-t172（各14.3%）。获得了7种不同的PFGE图谱。此外，耐药性最常见的是甲氧苄啶和青霉素（各71.4%），其次是红霉素（28.6%），氨苄青霉素，克林霉素和四环素（各14.3%）。所有菌株均对氯霉素、头孢西丁、苯唑西林、阿米卡星和万古霉素敏感。7株中有3株对3种或3种以上抗菌药物耐药。发现的抗性基因有：linA/linA '（100%）、blaZ（85.7%）、泰特（K）、ermC、ermT和ermB（各14.3%）。未检测到其它抗性基因。此外，最常鉴定的外毒素基因是seu、lukED、hla、hlb、hld（均为100%），其次是hlg和hlgv（均为85.7%）、lukPV（71.4%）、sea（57.1%）、see（42.9%）、etd（28.6%）以及seb、sec、sed、sej和sek（均为14.3%）。结果表明，S.金黄色葡萄球菌ST 6-t701在我国西北地区流行率较高，具有多重耐药特征，携带多种毒素和耐药基因。因此，应采取严格的卫生防护措施，避免沙门氏菌对食品的污染。金黄色葡萄球菌和毒素生产的食物。
The aim of the study was to investigate the molecular epidemiologic of Staphylococcus aureus isolated from seven staphylococcal food-poisoning (SFP) outbreaks between 2006 and 2013 in Xi'an, northwest China. A total of seven S. aureus isolates associated with seven SFP outbreaks were obtained and characterized by determining the multilocus sequence typing, spa typing, pulsed-field gel electrophoresis (PFGE), antimicrobial susceptibility, toxin and resistant genes. The results showed that S. aureus ST6-t701 (71.4%) was the most predominant clone, followed by ST5-t002 and ST59-t172 (each 14.3%). Seven different PFGE patterns were obtained. Moreover, resistance was most frequently observed to trimethoprim and penicillin (each 71.4%), followed by erythromycin (28.6%), ampicillin, clindamycin, and tetracycline (each 14.3%). All strains were susceptible to chloramphenicol, cefoxitin, oxacillin, amikacin, and vancomycin. Three of seven strains displayed resistance to three or more antimicrobials. Resistance genes were found as follows: linA/linA ' (100%), blaZ (85.7%), tet(K), ermC, ermT, and ermB (each 14.3%). Other resistant genes were not detected. In addition, the most frequently identified exotoxin genes were seu, lukED, hla, hlb, hld (each 100%), followed by hlg and hlgv (each 85.7%), lukPV (71.4%), sea (57.1%), see (42.9%), etd (28.6%), and seb, sec, sed, sej, and sek (each 14.3%). The results indicated that S. aureus ST6-t701, with a high prevalence in the northwest of China, exhibited multidrug resistance and harbored multiple toxin and resistance genes. Therefore, strict hygienic and preventative measures should be taken in order to avoid the contamination of food by S. aureus and toxin production in foods.