Identi?cation of a novel antisense RNA that regulates growth hormone receptor expression in chickens
Identi?cation of a novel antisense RNA that regulates growth hormone receptor expression in chickens
复制标题
鉴定出调节鸡生长激素受体表达的新型反义RNA
DOI：
10.1080/15476286.2019.1572440
复制
发表时间：
2019-05-04
期刊：
影响因子：
4.1
通讯作者：
Zhang, Xiquan
中科院分区：
文献类型：
--
作者：
Lin, Shudai;Zhang, Zihao;Zhang, Xiquan
天然反义转录物（NAT）广泛存在于哺乳动物基因组中，是基因表达的重要调控分子。然而，关于鸡中NAT的研究相对较少。我们确认了吗？艾德在鸡中发现了一种新的反义转录物，命名为GHR-AS-EST，其转录自生长激素受体（GHR）位点，该位点编码一种众所周知的肌肉发育和脂肪沉积的调节分子。GHR-AS-EST主要在鸡的肝脏和肌肉组织中表达。GHR-AS-EST序列在脊椎动物中保守性较弱。GHR-AS-EST与GHBP形成RNA-RNA双链体以增加其稳定性，并在mRNA和蛋白质水平上调节GHR正义转录本的表达。此外，在LMH肝细胞癌细胞中，GHR-AS-EST通过刺激JAK 2/STAT途径中的信号传导因子的表达来促进细胞增殖，并且通过下调JAK 2/SOCS途径中的信号传导因子的表达来促进脂肪沉积。我们期望发现与细胞增殖和脂解相关的调节基因的NAT将进一步加深我们对肌肉发育和脂肪沉积的分子调节的理解。
Natural antisense transcripts (NATs) are widely present in mammalian genomes and act as pivotal regulator molecules of gene expression. However, studies on NATs in the chicken are relatively rare. We identi?ed a novel antisense transcript in the chicken, designated GHR-AS-EST, transcribed from the growth hormone receptor (GHR) locus, which encodes a well-known regulatory molecule of muscle development and fat deposition. GHR-AS-EST is predominantly expressed in the chicken liver and muscle tissues. GHR-AS-EST sequence conservation among vertebrates is weak. GHR-AS-EST forms an RNA-RNA duplex with GHBP to increase its stability, and regulates the expression of GHR sense transcripts at both the mRNA and protein levels. Further, GHR-AS-EST promotes cell proliferation by stimulating the expression of signaling factors in the JAK2/STAT pathway, and contributes to fat deposition via downregulating the expression of signaling factors in the JAK2/SOCS pathway in LMH hepatocellular carcinoma cells. We expect that the discovery of a NAT for a regulatory gene associated with cell proliferation and lipolysis will further our understanding of the molecular regulation of both muscle development and fat deposition.