Localization and spatiotemporal expression of IDO following transient forebrain ischemia in gerbils
Localization and spatiotemporal expression of IDO following transient forebrain ischemia in gerbils
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DOI：
10.1016/j.brainres.2008.02.067
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发表时间：
2008-06-27
期刊：
影响因子：
2.9
通讯作者：
Mori, Hideki
中科院分区：
文献类型：
--
作者：
Taguchi, Ayako;Hara, Akira;Mori, Hideki
吲哚胺2，3-双加氧酶是犬尿氨酸途径中的限速酶，可将L-色氨酸转化为L-犬尿氨酸。短暂性前脑缺血引发一系列细胞事件，导致几个脑区迟发性神经元变性。本研究的目的是确定IDO在沙土鼠脑中的定位，并分析IDO在短暂性前脑缺血模型中的时空表达。用免疫组织化学方法观察IDO在正常沙土鼠脑中的表达。应用免疫组织化学方法观察短暂性前脑缺血沙土鼠脑内IDO表达的时程变化，并结合苏木精-伊红染色和原位末端脱氧核糖核酸缺口末端标记法(TUNEL)进行形态学分析。正常沙土鼠的丘脑、下丘脑和杏仁核有IDO免疫反应。IDO在扣带回皮质、海马CA1区和顶叶皮质呈阴性表达。短暂性脑缺血后，我们观察到IDO在CA1、扣带皮质和下丘脑的表达呈时间依赖性增加。IDO在CA1和扣带回的表达高峰出现在缺血后48h，2周后表达减弱。短暂性脑缺血后72h和96h仅在CA1区观察到TUNEL染色。因此，IDO蛋白存在于沙土鼠脑内特定的区域，并在短暂性脑缺血后的部分神经元中观察到IDO表达的动态变化。(C)2008爱思唯尔B.V.保留所有权利。
Indoleamine 2,3-dioxygenase (IDO) is the rate-limiting enzyme in the kynurenine pathway that converts L-tryptophan to L-kynurenine. Transient forebrain ischemia initiates a series of cellular events that lead to the delayed neuronal degeneration of several brain regions. The goal of this study was to determine the localization of IDO in gerbil brain, and analyze the spatiotemporal expression of IDO in a transient forebrain ischemic model. Expression of IDO in the normal gerbil brain was observed by using immunohistochemistry. Time-course of the expression of IDO following transient forebrain ischemic gerbils was examined by immunohistochemistry, combined with hematoxylin and eosin staining for morphological analysis, and in situ terminal dUTP-biotin nick end labeling of DNA fragments (TUNEL) method. In normal gerbils, IDO immunostaining was observed in thalamus, hypothalamus and amygdaloid nucleus. IDO expression was negative in the cingulate cortex, hippocampal CA1 region and parietal cortex. Following transient ischemia, we observed a time-dependent increase of IDO expression in CA1, cingulate cortex and hypothalamus. The peak of IDO expression in CA1 and cingulate cortex occurred at 48 h after ischemic insult and diminished by 2 weeks. TUNEL staining was observed only in the CA1 region at 72 and 96 h after transient ischemia. Thus, IDO protein is present in specific regions in gerbil brain, and dynamic changes of IDO expression was observed in some neurons following transient ischemia. (C) 2008 Elsevier B.V. All rights reserved.