Analogical China map constructed by single-stranded DNA tiles assembly
Analogical China map constructed by single-stranded DNA tiles assembly
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由单链DNA瓦片组装构建的类比中国地图
DOI：
10.1166/jctn.2016.5220
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发表时间：
2016-06
影响因子：
--
通讯作者：
Cui Guangzhao
中科院分区：
文献类型：
--
作者：
Wang Yanfeng;Ma Xin;Li Mengmeng;Pan Linqiang;Cui Guangzhao
在过去的近二十年里，DNA自组装技术作为一种很有前途的技术，在实验室的研究工作中层出不穷。单链DNA瓦片（SST）组装提供了一个简单的，模块化的和强大的框架，用于构建具有指定形状的纳米结构，从短的合成DNA链。在本研究中，我们提出了一个纳米级的DNA结构的类比中国地图，它是由一些SST构建的，并标记链霉亲和素在首都北京的位置。该纳米结构的直径约为98 nm × 67 nm，空间分辨率为3 nm × 7 nm。在原子力显微镜（AFM）中出现的图案符合预先设计的中国地图的形状，具有近似预期的尺寸，并且从云母中随机选择的场的AFM图像给出11%的组装产率。通过本研究，充分证明了SST构造复杂二维图形的能力和内部阵列的可控性。
In the past nearly two decades, DNA self-assembly technology as a promising technology, a body of laboratory work has been emerged in an endless stream. Single-stranded DNA tile (SST) assembly provides a simple, modular and robust framework for constructing nanostructures with prescribed shapes from short synthetic DNA strands. In this research, we present a nanoscale DNA structure of analogical China map which is constructed by a number of SSTs, and label streptavidin in the capital beijing location. The constructing nanostructure is roughly 98 nm × 67 nm in diameter with a spatial resolution of 3 nm × 7 nm. The pattern appeared in atomic force microscopy (AFM) conforms to the pre-designed shape of Chinese map with the approximately expected dimensions, and AFM images of the field selected randomly from the mica give an 11% assembly yield. Through this research, the capability of constructing complicated two-dimensional shapes by SST and the controllability of the internal array are fully vindicated.