K562 cell anion exchange differs markedly from that of mature red blood cells.
K562 cell anion exchange differs markedly from that of mature red blood cells.
复制标题
K562 细胞阴离子交换与成熟红细胞的阴离子交换显着不同。
DOI：
10.1152/ajpcell.1983.244.1.c68
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发表时间：
1983
期刊：
影响因子：
--
通讯作者：
Knauf,PA
中科院分区：
文献类型：
--
作者：
Law,FY;Steinfeld,R;Knauf,PA
人K562白血病细胞表现出几种红细胞特性，包括主要红细胞唾液酸糖蛋白血型糖蛋白的合成和表达。这使我们想知道，这些细胞是否表达一种功能性阴离子转运系统，类似于与另一种主要红细胞糖蛋白带3相关的系统。K562细胞中的氯-36交换通量小于在类似条件下成熟红细胞中预期的氯-36交换通量的0.6%。与红细胞不同，K562细胞不表现出高的氯化物-硫酸盐选择性，并且抑制红细胞氯化物交换的各种试剂在K562细胞中的效果都要差得多。基于这些通量测量，K562细胞每个细胞可能含有少于600个完全功能的红细胞样带3分子，而成熟红细胞中约有一百万个分子。不太可能存在转换率降低和/或对氯化物和各种抑制剂亲和力降低的带3分子发生极大改变的可能性，但不能完全排除。阴离子转运也在K562细胞中进行了测量，这些细胞已被各种化学试剂诱导增加血红蛋白合成。即使在这些条件下，氯离子通量表明功能阴离子转运位点或其氯离子转运速率的数量没有实质性增加。
Human K562 leukemic cells exhibit several erythroid properties, including synthesis and expression of the major red blood cell sialoglycoprotein, glycophorin. This has led us to ask if these cells express a functional anion transport system analogous to that which is associated with the other major erythrocyte glycoprotein, band 3. The chloride-36 exchange flux in K562 cells is less than 0.6% of that which would be expected in mature erythrocytes under similar conditions. Unlike red blood cells, K562 cells do not exhibit a high chloride-sulfate selectivity, and various agents that inhibit red blood cell chloride exchange are all much less effective in K562 cells. On the basis of these flux measurements, K562 cells probably contain less than 600 fully functional red blood cell-like band 3 molecules per cell, in contrast to about a million molecules in the mature red blood cell. The possible-existence of greatly altered band 3 molecules with a reduced turnover rate and/or a reduced affinity for chloride and for various inhibitors is unlikely but cannot be completely excluded. Anion transport was also measured in K562 cells that had been induced to increase hemoglobin synthesis by various chemical agents. Even under these conditions, chloride fluxes indicated no substantial increase in the number of functional anion transport sites or their chloride transport rate.